Journal of Oral Implantology February 2013 - (Page 14)

REGULAR ARTICLE Evaluation of the Bone Healing Process Utilizing PlateletRich Plasma Activated by Thrombin and Calcium Chloride: A Histologic Study in Rabbit Calvaria Walter Betoni-Junior, DDS, MSc, PhD1 Paula Dechichi, DDS, MSc, PhD2 ˆ Jonatas Caldeira Esteves, DDS, MSc1* Darceny Zanetta-Barbosa, DDS, MSc, PhD3 ´ ´ ˜ Aparecido Eurıpedes Onorio Magalhaes, DDS, MSc, PhD3 To evaluate the bone healing of defects filled with particulate bone graft in combination with platelet-rich plasma (PRP), added with a mixture of calcium chloride and thrombin or just calcium chloride. Two 5-mm bone defects were created in the calvaria of 24 rabbits. Each defect was filled with particulate bone graft and PRP. In one defect the PRP was activated by a mixture of calcium chloride and thrombin; in the other, PRP was activated by calcium chloride only. The animals were euthanized 1, 2, 4, and 8 weeks after the surgeries, and the calvaria was submitted to histologic processing for histomorphometric analysis. The qualitative analysis has shown that both defects presented the same histologic characteristics so that a better organized, more mature, and wellvascularized bone tissue was noticed in the eighth week. A good bone repair was achieved using either the mixture of calcium chloride and thrombin or the calcium chloride alone as a restarting agent of the coagulation process. Key Words: platelet-rich plasma, bone graft, dental implants, growth factors INTRODUCTION D eveloped in the last decade from fibrin adhesives, PRP, or platelet-rich plasma, has been widely studied and applied for tissue repair. It is an autologous source of platelet-derived growth factors which aim to stimulate or accelerate the healing process.1,2 In implantology and oral and maxillofacial surgery and traumatology, its main application is 1 Division of Oral and Maxillofacial Surgery, Department of Surgery and Integrated Clinic, Dental School of Aracatuba, ¸ UNESP - Univ Estadual Paulista, Aracatuba, Brazil. ¸ 2 Department of Morphology, Area of Histology, Division of Basic ˆ ˆ Sciences, Federal University of Uberlandia, Uberlandia, Brazil. 3 Department of Oral & Maxillofacial Surgery and Implantology, ˆ ˆ Federal University of Uberlandia, Uberlandia, Brazil. * Corresponding author, e-mail: jonatasce@hotmail.com DOI: 10.1563/AAID-JOI-D-10-00043 14 Vol. XXXIX /No. One /2013 related to the bone repair process, especially in reconstructive procedures.3,4 The addition of PRP to autogenous bone grafts or biomaterials has been showing promising results both concerning the speed5 and the bone neoformation quality.6–8 These characteristics are based upon the liberation of growth factors contained in the platelets’ alpha granules, such as PDGF, PGF-b, PDEGF, IGF, and PF4, each of them with different activities related to the increase of chemotaxis for undifferentiated mesenchymal cells, macrophages and osteoblasts, stimulus for fibroblasts and osteoblast differentiation and proliferation, collagenous matrix secretion, angiogenesis, and deposition of mineralized tissue.9,10 PRP preparation is accomplished through venous punch and autologous blood collection in Vacutainer tubes containing the anticoagulant sodium citrate. After a centrifugation process, PRP

Table of Contents for the Digital Edition of Journal of Oral Implantology February 2013

Could the Fountain of Youth Be All in Your Bones?
Vertical Bone Augmentation With Simultaneous Implant Placement Using Particulate
Evaluation of the Bone Healing Process Utilizing Platelet-Rich Plasma Activated by Thrombin
Effect of Model Parameters on Finite Element Analysis of Micromotions in Implant Dentistry
Peri-Implant Defect Augmentation With Autogenous Bone: A Study in Beagle Dogs
Would Nitric Oxide be an Effective Marker for Earlier Stages of Peri-Implant Disease? An
The Effect of Different Surface Treatments on Cement-Retained Implant-Supported
Effect of Rotating Osteotomes on Primary Implant Stability—An In Vitro Investigation
Horizontal Augmentation Through the Ridge-Split Procedure: A Predictable Surgical
The Bis-Acryl Stent
Clinical, Histological, and Histomorphometrical Analysis of Maxillary Sinus Augmentation
A Technique to Salvage a Single Implant-Supported Fixed Dental Prosthesis Having a
Ridge Expansion and Immediate Placement With Piezosurgery and Screw Expanders in
Pterygoid Implants for Maxillofacial Rehabilitation of a Patient With a Bilateral Maxillectomy
Technology in Maxillary Premolar Region: A New Strategy for Soft Tissue Management
Fracture of Anterior Iliac Crest Following Bone Graft Harvest in an Anorexic Patient: Case
A Technique for Constructing a New Maxillary Overdenture to a Nonretrievable Implant
Edentulous Maxillary Arch Fixed Implant Rehabilitation Using a Hybrid Prosthesis Made of

Journal of Oral Implantology February 2013