Journal of Oral Implantology April 2013 - (Page 154)
RESEARCH
Fluoride-Treated Bio-Resorbable Synthetic
Hydroxyapatite Promotes Proliferation and
Differentiation of Human Osteoblastic MG-63 Cells
Motofumi Ohno, DDS1
Kazunari Kimoto, DDS, PhD1*
Toshihisa Toyoda, DDS, PhD2
Kazushige Kawata, DDS1
Hirohisa Arakawa, DDS, PhD1
When resorbable hydroxyapatite (HA) granules, which are used as a bone supplement material, were treated in
neutral 4% sodium fluoride (NaF) solution, formation of a reactant resembling calcium fluoride was observed on
the surface of the granules. Immediate and slow release of fluoride from fluoridated HA (HAĆ¾F) granules was
observed after immersion in culture fluid, and the concentration increased over time to 1.25 6 0.05 ppm F at 0.5
hours, 1.57 6 0.12 ppm F at 24 hours, and 1.73 6 0.15 ppm F at 48 hours. On invasion assay, migration of
human osteoblast-like MG-63 cells exposed to the released fluoride was confirmed in comparison to the cells
incubated with a nonfluoridated control sample (P , .01). In addition, fluoride added to the medium increased
MG-63 cell proliferation in a manner dependent on fluoride concentrations up to 2.0 ppm (P , .05). At 5.0 ppm,
however, fluoride significantly inhibited cell proliferation (P , .005). Activity of the osteogenic differentiation
marker, alkaline phosphatase (ALP), also increased with fluoride after exposure for 1 week, increasing
significantly at 1.0 ppm (P , .05). The promotion of MG-63 cell migration and proliferation, as well as increased
ALP activity, suggested that fluoride released from the surface of resorbable HA granules, which were
fluoridated by prior treatment with neutral 4% NaF solution, can provide a superb method to supply fluoride
and promote osteogenic cell differentiation.
Key Words: fluoride slow release, bio-resorbable hydroxyapatite, cell migration, proliferation, ALP activity
INTRODUCTION
I
mplants cannot be placed in deficient bone
and pretreatments such as guided bone
regeneration are necessary in such cases.
Materials currently used for guided bone
regeneration include autograft bone, allograft
bone, xenogeneic bone, and artificial materials such
as hydroxyapatite (HA) and tricalcium phosphate
granules.1,2
1
Division of Oral Health, Department of Health Science,
Kanagawa Dental College, Yokosuka, Japan.
Tokyo Plastic Surgery Dental Society, Tokyo, Japan.
* Corresponding author, e-mail: kimotokz@kdcnet.ac.jp
DOI: 10.1563/AAID-JOI-D-10-00175
2
154
Vol. XXXIX /No. Two / 2013
In a previous study, fluorapatite was prepared
from resorbable synthetic HA granules by treating
them with 4% sodium fluoride (NaF) at neutral pH
for 2 minutes. Rinsed powder was then subjected to
analyses using electron microscopy, 1-dimensional
and 2-dimensional X-ray diffraction, energy dispersive X-ray (EDX) analysis, and EDX mapping.3 We
showed that the reaction of fluoride ions with the
HA granules produced crystalline fluorapatite having characteristic and sharp X-ray diffraction patterns on the surface of granules. EDX analysis
revealed a fluoride peak at 0.70 KeV that was not
seen on the surface of nonfluoridated control
granules. Furthermore, EDX mapping showed an
evenly distributed needle crystalline particulate
Table of Contents for the Digital Edition of Journal of Oral Implantology April 2013
Evidence-Based Practice or Practice-Based Evidence?
Evaluation of Optimal Taper of Immediately Loaded Wide-Diameter Implants: A Finite Element Analysis
A Prospective, Randomized Controlled Preclinical Trial to Evaluate Different Formulations of Biphasic Calcium Phosphate in Combination With a Hydroxyapatite Collagen Membrane to Reconstruct Deficient Alveolar Ridges
Retention of Implant-Supported Overdenture With Bar/Clip and Stud Attachment Designs
The Clinical and Histologic Outcome of Dental Implant in Large Ridge Defect Regenerated With Alloplast: A Randomized Controlled Preclinical Trial
Fluoride-Treated Bio-Resorbable Synthetic Hydroxyapatite Promotes Proliferation and Differentiation of Human Osteoblastic MG-63 Cells
Resonance Frequency Analysis of 208 Straumann Dental Implants During the Healing Period
A Simplified Technique for Solving the Transfer Problem of Implant-Supported Fixed Partial Dentures for Patients With Microstomia
Esthetic Evaluation of Single-Tooth Morse Taper Connection Implants Placed in Fresh Extraction Sockets or Healed Sites
A Technique for Improving the Retention of Dentures Retained by Ramus Frame Implants
Treatment of Congenitally Missing Maxillary Lateral Incisors: An Interdisciplinary Approach
Use of Titanium Mesh in Lieu of a Fixation Screw to Stabilize an Autogenous Block Graft: A Case Report
A Novel Approach to Preserve the Buccal Wall in Immediate Implant Cases: A Clinical Report
Implant Failure Associated With Actinomycosis in a Medically Compromised Patient
Replacement of Mandibular Posterior Teeth With Implants in a Postmandibular Resection Case: A Case Report
Zygomatic Implants/Fixture: A Systematic Review
An Overview of Immediate Root Analogue Zirconia Implants
Letter to the Editor
Reviews of Current Literature
Journal of Oral Implantology April 2013
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