Journal of Oral Implantology August 2013 - (Page 433)
RESEARCH
Autologous Dental Pulp Stem Cells in Regeneration of
Defect Created in Canine Periodontal Tissue
Afshin Khorsand, DMD, MS1
Mohamadreza Baghaban Eslaminejad, PhD2*
Mohadeseh Arabsolghar, DMD, MS3
Mojghan Paknejad, DMD, MS1
Baharak Ghaedi, DMD, MS4
Amir Reza Rokn, DMD, MS1
Neda Moslemi, DMD, MS1
Hamid Nazarian, MSc2
Shahrbanoo Jahangir, MSc2
This study aimed to investigate effects of dental pulp stem cells (DPSCs) on regeneration of a defect experimentally
created in the periodontium of a canine model. Surgically created mesial 3-walled periodontal defects with ligatureinduced periodontitis were produced bilaterally in the first lower premolar teeth of 10 mongrel dogs. Simultaneously,
DPSCs were derived from the maxillary premolar teeth of the same dogs. Four weeks after creation of the
periodontitis model, autologous passaged-3 DPSCs combined with Bio-Oss were implanted on one side as the test
group. On the other side, only Bio-Oss was implanted as a control. Eight weeks after surgery, regeneration of the
periodontal defects was evaluated histologically and histomorphometrically in terms of bone, periodontal ligament
(PDL), and cement formation. Histologically, in all test specimens (10 defects), regeneration of cementum, bone, and
PDL was observed. In the control groups, although we observed the regeneration of bone in all defects, the formation
of cementum was seen in 9 defects and PDL was seen in 8 defects. Histomorphometric analyses showed that the
amount of regenerated cementum and PDL in the test groups (3.83 6 1.32 mm and 3.30 6 1.12 mm, respectively)
was significantly higher than that of the control groups (2.42 6 1.40 mm and 1.7761.27 mm, respectively; P , .05). A
biocomplex consisting of DPSCs and Bio-Oss would be promising in regeneration of periodontal tissues.
Key Words: dental pulp stem cells, regeneration, periodontal defect, Bio-Oss
INTRODUCTION
P
1
eriodontitis is a complex disease of the
periodontium that results in considerable defects to alveolar bone, gingival
tissue, and periodontal ligament (PDL).1
Reconstruction of defects resulting from
Dental Research Center, Periodontics Department, Faculty of
Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
2
Department of Stem Cells and Developmental Biology at Cell
Science Research Center, Royan Institute for Stem Cell Biology
and Technology, ACECR, Tehran, Iran.
3
Department of Periodontology, School of Dentistry, Kerman
University of Medical Sciences. Kerman, Iran.
4
Department of Periodontology, School of Dentistry, Hamedan
University of Medical Sciences. Hamedan, Iran.
* Corresponding author, e-mail: eslami@royaninstitute.org
DOI: 10.1563/AAID-JOI-D-12-00027
periodontal disease is a major challenge to regenerative medicine in the oromaxillofacial field. On the
other hand, in the field of tissue regeneration, the
use of tissue-resident stem cells as regenerative
materials has gained considerable attention owing
to their self-renewal property and multilineage
differentiation capacity.
Among stem cells, dental stem cells are of
interest as they are easily accessible and can be
collected noninvasively with low morbidity.2 In this
context, the periodontal ligament stem cell (PDLSC)
has been successfully investigated in some animal
models. Liu et al3 investigated the utility of
autologous PDLSC to treat ligature-induced periodontitis in miniature swine. The 3-walled defect
was generated in the first molar area of miniature
Journal of Oral Implantology
433
Table of Contents for the Digital Edition of Journal of Oral Implantology August 2013
Literary Responsibility—Everyone Has a Role to Play
Rehabilitation of Surgically Relocated Integrated Dental Implants With and Without Bone Morphogenesis Protein-2
Erratum
Marginal Fit of Implant-Supported All-Ceramic Zirconia Frameworks
Influence of the Implant Diameter With Different Sizes of Hexagon: Analysis by 3-Dimensional Finite Element Method
Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue
Histologic and Biomechanical Evaluation of 2 Resorbable-Blasting Media Implant Surfaces at Early Implantation Times
Efficacy of Guided Bone Regeneration Using Composite Bone Graft and Resorbable Collagen Membrane in Seibert’s Class I Ridge Defects: Radiological Evaluation
Minimal Iliac Bone Harvesting in Sinus Lift Surgery
Functional Load in Oblique Bicortical Implants: Parasinusal Implants and Palatine Implants
Ultrasonic Oscillations for Conservative Retrieval of a Rare Fracture of Implant Healing Abutment
Craniofacial Implant-Retained Auricular Prosthesis: A Case Report
Gingival Conditioning in an Implant-Supported Prosthesis: A Clinical Report
An Early Loaded Implant-Supported Mandibular Complete Arch Fixed Prosthesis in a Young Completely Edentulous Patient: A Case Report
Computer Guided Surgery for Implant Placement and Dental Rehabilitation in a Patient Undergoing Sub-Total Mandibulectomy and Microvascular Free Flap Reconstruction
Guided Bone Regeneration for Socket Preservation in Molar Extraction Sites: Histomorphometric and 3D Computerized Tomography Analysis
Osseointegration of Dental Implants and Osteonecrosis of the Jaw in Patients Treated With Bisphosphonate Therapy: A Systematic Review
Review of Current Literature
Journal of Oral Implantology August 2013
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