Journal of Oral Implantology December 2013 - (Page 635)

RESEARCH Effect of Different Storage Media on the Regenerative Potential of Autogenous Bone Grafts: A Histomorphometrical Analysis in Rabbits Flaviana Soares Rocha, MD1* Jonas Dantas Batista, MD1 Darceny Zanetta-Barbosa, PhD1 Paula Dechichi, PhD2 The success of autogenous bone graft is related to the graft cell viability. In bone-grafting procedures, harvested grafts are often maintained in extraoral media while the recipient site is prepared. The aim of this study was to evaluate in vivo the effect of storage media over autogenous bone grafts during the transsurgical time. Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was immediately fixed in the mandibular angle (control group), and the other was maintained in air exposure (dry group), 0.9% NaCl solution (saline group), or platelet-poor plasma (PPP group) during 30 minutes and stabilized in the symmetrical location of control grafts. After 28 days, the animals were euthanized and the bone fragments were removed, demineralized, and embedded in paraffin. Histological evaluation was performed under light microscope. Empty lacunae and bone graft area quantification were carried out for the sections. The histomorphometrical analysis revealed reduction of the graft area and increase of empty lacunae in the dry group when compared with control. No significant differences were found in the number of empty lacunae or bone graft area between the saline group and its control and also between the PPP group and its control. The dry group showed more empty lacunae and less graft area than the saline and PPP groups. In accordance with the results, PPP and physiologic solution demonstrated osteocyte preservation and bone graft area maintenance, being satisfactory storage media for autogenous bone grafts during the transsurgical period. Key Words: osteocyte lacunae, bone graft, platelet-poor plasma INTRODUCTION B one grafts are frequently used for treatment of severe bone loss.1-3 Autogenous bone grafts are more predictable when compared with other biomaterials because of their biological properties,4 and they are considered the gold standard for bone reconstruction.1,2,4 Graft volume 1 School of Dentistry, Oral & Maxillofacial Surgery and Implanˆ tology Department, University of Uberlandia, Minas Gerais, Brazil. 2 ˆ Biomedical Science Institute, University of Uberlandia, Minas Gerais, Brazil. * Corresponding author, e-mail: flavianasoares.rocha@gmail.com DOI: 10.1563/AAID-JOI-D-11-00020 maintenance and early bone remodeling are important factors in the success of these surgical procedures.5 During a bone-grafting procedure, there is usually a delay between removal of the graft and its stabilization. Preparation of the recipient sites may sometimes begin after the donor site has been surgically closed.6 This raises several questions concerning the harmful effects of this delay over osteogenesis and the optimal environment for the graft.7,8 Experimental evidence demonstrates that the living cells within a fresh bone graft contribute to osteogenesis within and around the graft.2 If the Journal of Oral Implantology 635

Table of Contents for the Digital Edition of Journal of Oral Implantology December 2013

Blade-Form Dental Implants: FDA Reclassification as a Class II Dental Implant Device
Effect of Different Storage Media on the Regenerative Potential of Autogenous Bone Grafts: A Histomorphometrical Analysis in Rabbits
Tensile Resistance of Mineralized and Demineralized Rat Bones in Different Regions (Calvarial and Femur)
Microbial Diversity of Peri-Implant Biofilms on Implant Fixed Bar and Telescopic Double Crown Attachments
Evidence-Based Techniques to Assess the Performance of Dental Implants
Influence of Platform and Abutment Angulation on Peri-Implant Bone. A Three- Dimensional Finite Element Stress Analysis
Direct Current Electric Stimulation in Implant Osseointegration: An Experimental Animal Study With Sheep
Prevalence of Sinus Augmentation Associated With Maxillary Posterior Implants
The Bactericidal Effects of an Acidified Sodium Chlorite-Containing Oral Moisturizing Gel: A Pilot Study
Influence of Fine Threads and Platform-Switching on Crestal Bone Stress Around Implant— A Three-Dimensional Finite Element Analysis
An Evaluation of the Resonance Frequency Analysis Device: Examiner Reliability and Repeatability of Readings
Histologic and Histomorphometric Assessment of Implants and Periapical Tissues When Placed in the Sockets of Extracted Teeth, Teeth With Periapical Lesions, and Healed Lesions: A Canine Study
Microbiological Aspects of Human Mandibular Subperiosteal Dental Implants
A Technique for Removal of a Fractured Implant Abutment Screw
Eight-Year Results of Site Retention of Anorganic Bovine Bone and Anorganic Bovine Matrix
A Reactive Lesion (Pyogenic Granuloma) Associated With Dental Implant: A Case Report
Twelve-Year Clinical and Radiological Results of Maxillary and Mandibular Implant- Retained Bar Overdentures Carried out on Oxidized (TiUnite) Replace Select Implants: A Clinical Case
PEEK Dental Implants: A Review of the Literature

Journal of Oral Implantology December 2013