Journal of Oral Implantology December 2014 - (Page 655)

RESEARCH Role of rhBMP-2 and rhBMP-7 in the Metabolism and Differentiation of Osteoblast-Like Cells Cultured on Chemically Modified Titanium Surfaces Fabiano Ribeiro Cirano, PhD1* Adriane Yaeko Togashi, PhD2 ´ Marcia Martins Marques, PhD3 ´ Francisco Emılio Pustiglioni, PhD4 ˆ Luiz Antonio Pugliesi Alves Lima, PhD4 This study analyzed the role of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human bone morphogenetic protein 7 (rhBMP-7) in the adhesion and differentiation of rat osteoblast-like (osteo-1) cells cultured on chemically modified titanium surfaces. Osteo-1 cells were cultured on chemically modified (modified sandblasted and acid-etched) titanium surfaces in 3 different types of medium: control, medium supplemented with 20 ng/mL rhBMP-2, and medium supplemented with 20 ng/mL rhBMP-7. The following parameters were evaluated: cell adhesion after 24 hours; total protein content; collagen content and alkaline phosphatase (AP) activity after 7, 14, and 21 days; and calcified nodule formation after 21 days. The addition of rhBMP-2 or rhBMP-7 did not influence cell adhesion (P ¼ .1175). Cell differentiation was influenced by rhBMP-2, as demonstrated by a significant increase in collagen content after 7 days of culture (P , .0001) and a significant decrease in AP activity after 21 days (P , .0001). The addition of rhBMP-7 only influenced AP activity, and a significant increase was observed after 21 days (P , .0001). Within the limitations of the study, we conclude that the presence of rhBMP-2 or rhBMP-7 did not influence cell adhesion to chemically modified titanium surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface. Key Words: bone morphogenetic protein, titanium surface, implants, osteoblasts INTRODUCTION C 1 hemical modification of titanium surfaces may stimulate osteoblast function, improving the adhesion and differentiation of osteoblasts cultured on these surfaces. Modified sandblast- ˜ Paulista University, Department of Periodontology, Sao Paulo, Brazil. 2 Oeste University, Department of Periodontology and Implan´ tology, Parana, Brazil. 3 ˜ ˜ University of Sao Paulo, Department of Dentistry, Sao Paulo, Brazil. 4 ˜ ˜ University of Sao Paulo, Department of Periodontology, Sao Paulo, Brazil. * Corresponding author, e-mail: e-mail: cirano@unip.br DOI: 10.1563/AAID-JOI-D-12-00071 ed and acid-etched (modSLA) titanium surfaces present a change in their reactivity characterized by a substantial increase in their hydrophilic character.1,2 This surface may promote a better boneimplant contact during the first weeks of osseointegration, favoring faster bone formation. In parallel, studies have shown that bone morphogenetic proteins (BMPs) are extremely important for the process of bone formation.3 In addition, BMPs, especially recombinant human bone morphogenetic protein 2 (rhBMP-2), play a crucial role in the proliferation and differentiation of osteoblasts.4-6 Another relevant BMP, recombinant human bone morphogenetic protein 7 (rhBMP-7), has the capacity to stimulate bone metabolism, Journal of Oral Implantology 655

Table of Contents for the Digital Edition of Journal of Oral Implantology December 2014

Effect of Splinting in Accuracy of Two Implant Impression Techniques
Assessment of the Stress Transmitted to Dental Implants Connected to Screw-Retained Bars Using Different Casting Techniques
Retentiveness of Various Luting Agents Used With Implant-Supported Prosthesis: An In Vitro Study
Role of rhBMP-2 and rhBMP-7 in the Metabolism and Differentiation of Osteoblast-Like Cells Cultured on Chemically Modified Titanium Surfaces
Coated vs Uncoated Implants: Bone Defect Configurations After Progressive Peri-implantitis in Dogs
Why Guided When Freehand Is Easier, Quicker, and Less Costly?
Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory Cells
Flapless Alveolar Ridge Preservation Utilizing the ‘‘Socket-Plug’’ Technique: Clinical Technique and Review of the Literature
Implants Placed in the Nasopalatine Canal to Rehabilitate Severely Atrophic Maxillae: A Retrospective Study With Long Follow-up
Severe Mandibular Atrophy Treated With a Subperiosteal Implant and Simultaneous Graft With rhBMP-2 and Mineralized Allograft: A Case Report
Full-Mouth Rehabilitation of a Patient With Ectodermal Dysplasia With Dental Implants
The 2-Visit CAD-CAM Implant-Retained Overdenture: A Clinical Report
Assessment of Implant-Related Treatment With Edited Three-Dimensional Reconstructed Images From Cone-Beam Computerized Tomography: A Technical Note
Retrieval of a Separated Implant Screwdriver Fragment
Correction of Esthetic Complications of a Malpositioned Implant: A Case Letter
Fixed Partial Denture Treatment With Mini Dental Implants
Replacement of an Implant and Prosthesis in the Premaxilla Due to a Malposition and Prosthetic Failure: A Clinical Case Letter
A New Restorative Technique for the Perishing Implant Due to Abutment Screw Fracture
Fit of CAD/CAM Implant Frameworks: A Comprehensive Review

Journal of Oral Implantology December 2014