CES - Revista Veterinaria Julio - Diciembre 2013 - (Page 52)

Evaluation of screening tests for antimicrobial residues in milk from individual cows treated with a combination of penicillins G and streptomycin* Valoración de pruebas de campo para detección rápida de residuos antimicrobianos en leche individual de vacas tratadas con una combinación de penicilinas y estreptomicina Valoração de testes de campo para detecção rápida de resíduos antimicrobianos em leite individual de vacas tratadas com uma mistura de penicilinas e estreptomicina Violeta Díez 1*, Zoot; Juan E. Pérez 1, MSc; Martha Olivera1, MV, MSc, PhD; Juan G. Restrepo 1, MV, PhD; David Villar1, MV, MSc, PhD. * Corresponding autor: Violeta Diez BIOGENESIS Research Group, Faculty of Agricultural Sciences, Universidad de Antioquia, AA 1226, Medellín, Colombia. Email: violetadb@hotmail.com BIOGÉNESIS Research Group, Faculty of Agricultural Sciences, Universidad de Antioquia, AA 1226, Medellín, Colombia. 1 (Recibido: 17 de octubre, 2013; aceptado: 22 de noviembre, 2013) Abstract Background: in Colombia, the law (Resolution 1382, 2013) prohibits the sale of milk that contains any antimicrobial drug residues, although no specific official screening tests and detection limits have been specified. At present, milk with positive results to both the Delvotest® and Snap® assays is simply rejected. To avoid contaminating bulk tanks with milk from individually treated cows, producers would benefit from having on-farm screening tests to conduct their own quality controls. In addition, on-site testing would allow farmers to check if the withdrawal times of commercially-available generic products are in accordance with labeled recommendations. Material and Methods: In this study, two commonly used rapid detection tests (Delvotest® and SNAP® specific for beta-lactams) were used on milk from 39 subclinical mastitic Holstein cows that were prescribed with daily intramuscular injections of a commercial suspension containing 8.000.000 IU of penicilin G (75% procaine penicilin G, 25% potasium penicillin) and 8 g of streptomycin sulfate, for a total of 4 days. Cows were individually milked and samples collected every 12 hours the day before, and for 3 days after the recommended withdrawal time of three days post-treatment. To inactivate the potential action of natural inhibitors of microbial growth that may be present in milk (ie., lysozyme and lactoferrin), the results of the Delvotest® were compared before and after milk samples were subjected to heat treatment (82°C for 5 minutes). Results: When the Delvotest® was used as per manufacturer's instructions (i.e., without heating), 7 of 39 cows were positive for one more day past the recommended withdrawal period. However, *Para citar este artículo: Díez V, Pérez JE, Olivera Ángel M, Restrepo JG, Villar D. Evaluation of screening tests for antimicrobial residues in milk from individual cows treated with a combination of penicillins G and streptomycin. Rev CES Med Zootec. 2013; Vol 8 (2): 52-60. 52 Revista CES Medicina Veterinaria y Zootecnia / Volumen 8 / Número 2 / julio - diciembre de 2013/ ISSN 1900-9607 http://www.revistamvzces.com/revistas/vol8no2/Articulo_4.pdf

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CES - Revista Veterinaria Julio - Diciembre 2013
Editorial: Bienestar animal: más que una moda
Producción de metano in vitro de dos gramíneas tropicales solas y mezcladas con Leucaena leucocephala o Gliricidia sepium
Expresión molecular de la vilina en yeyuno de lechones posdestete que consumieron LPS de E. coli
Cinética de degradación ruminal del ensilaje de maíz con diferentes niveles de inclusión de vinaza
Evaluation of screening tests for antimicrobial residues in milk from individual cows treated with a combination of penicillins G and streptomycin*
Inmunolocalización hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
Diseño y evaluación de una prueba de PCR para detección de Brucella spp. y Brucella abortus
Laparoscopia versus laparotomía paraprepucial en el tratamiento de criptorquidismo abdominal en caninos
ELISA indirecta para el diagnóstico de fasciolosis bovina en leche
Evaluación serológica de Leptospira interrogans en equinos pertenecientes a un centro ecuestre de la provincia de Linares, Chile
Inactivación del cromosoma X en el desarrollo embrionario mamífero
Coçar-se, limpar-se e estabelecer vínculos sociais: o “grooming” e seu significado biológico nos ruminantes
Criopreservação de sêmen suíno: avanços tecnológicos e perspectivas
Metaplasia ósea en el músculo flexor digital profundo en un Mastín Napolitano
Índice del Volumen 8, 2013 - Índice de Autores
Evaluadores Volumen 8, 2013

CES - Revista Veterinaria Julio - Diciembre 2013