LCGC The Peak - April 2008 - (Page 15)

APRIL 2008 THE PEAK 15 achieved. For example, proteins or peptides can be separated with SEC in the first dimension and reversed-phase LC in the second. The aqueous mobile phase used in the first dimension is a very weak eluent in reversedphase LC, causing effective focusing at the top of the second-dimension column. The availability of suitable software for data acquisition, data manipulation (“demodulation”), display, and quantitative analysis is still a bit of a bottleneck, but one that now appears to be fading rapidly. Several vendors now offer software packages that perform the most important tasks adequately. This allows scientists to focus on the more important data-interpretation aspects. A good deal of effort is now being devoted to the development and application of advanced data-analysis strategies (“chemometrics”) to comprehensive two-dimensional chromatography. One eye-catching example is the quality control of natural products, such as traditional Chinese medicines, using fingerprints obtained by LC LC. Another important application of LC LC can be in the search for biomarkers, based upon approaches from systems biology. This is one example in which LC LC–MS is obviously needed. There are no fundamental problems associated with the realization of such advanced systems. However, practical problems emerge from the required (scanning) speed and from the sheer amounts of data generated during each run. Finally, having mentioned systems biology, it is worth mentioning that despite the great potential of LC LC separating, for example, all proteins in the human proteome is still not within our grasp. We are starting to play with ideas for realizing comprehensive threedimensional LC (LC LC LC). It is good to know that liquid chromatographers still have major challenges ahead of them. References (1) J.B. Philillips, J. Xu, J.Chromatogr. 703, 327 (1995). (2) F. Erni and R.W. Frei, J.Chromatogr. 149, 561 (1978). (3) D.R. Stoll, J.D. Cohen, and P.W. Carr, J.Chromatogr., A 1122, 123 (2006). (4) P.J. Schoenmakers and P. Marriott, J. Beens, LCGC Eur. 16, 335 (2003). (5) A. van der Horst and P.J. Schoenmakers, J.Chromatogr., A 1000, 693 (2003). (6) M.R. Schure, Anal.Chem. 71, 1645 (1999). (7) K. Horie, H. Kimura, T. Ikegami, A. Iwatsuka, N. Saad, N., O. Fiehn, and N. Tanaka, Anal.Chem. 79, 3764 (2007). (8) D.R. Stoll, X. Li, X. Wang, P.W. Carr, S.E.G. Porter, and S. C. Rutan, J.Chromatogr., A 1168, 3 (2007). (9) U.D. Neue, J.Chromatogr., A, in press. (10) X. Wang, D.R. Stoll, P.W. Carr, and P.J. Schoenmakers, J.Chromatogr., A 1125, 177 (2006). (11) Q. Luo, Y. Shen, K.K. Hixson, R. Zhao, F. Yang, and R.J. Moore, Anal.Chem. 77, 5028 (2005). (12) J. Billen and G. Desmet, J. Chromatogr., A 1168, 73 (2007). (13) P.J. Schoenmakers, G. Vivoì-Truyols and W.M.C. Decrop, J.Chromatogr., A 1120, 282 (2006). (14) J.C. Giddings, J.Chromatogr., A 703, 3 (1995). (15) P. Dugo, F. Cacciola, Tiina Kumm, G. Dugo, and L. Mondello, J.Chromatogr., A, in press.

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LCGC The Peak - April 2008 Contents LC3LC: Comprehensive Two-Dimensional Liquid Chromatography Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns Industry News Events Education

LCGC The Peak - April 2008

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