LCGC The Peak - April 2008 - (Page 20) 20 THE PEAK APRIL 2008 A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns Naoto Furusawa, Graduate School of Human Life Science, Osaka City University, Osaka, Japan. A simple and inexpensive sample preparation method followed by reversed-phase high performance liquid chromatography (RP-HPLC) was developed to quantify oxytetracycline (OTC), chlortetracycline (CTC), and tetracycline (TC) residues in prawns. The total analytical time, including sample preparation, was 15 min for each sample and no organic solvents were used in the sample preparation or chromatography stages. T etracycline antibiotics (TCAs) are used globally in aquaculture to control disease and promote growth. The tetracyclines used in veterinary medicine in Japan account for more than half the total of antibiotics used (1). The overuse of TCAs can result in their presence in seafood for human consumption. This is a serious problem in Japan where seafood is consumed in copious amounts and prawns are the Japanese consumer’s favourite choice. A report that 2.3 ppm of oxytetracycline (OTC) was detected in imported Japanese Tiger Prawn (Marsupenaeus japonicus) magnified these concerns. This level is greater than the minimal risk level (MRL) of 0.1 g/g in fish — singly or in combination — the EU has set for OTC, chlortetracycline (CTC) and tetracycline (TC) (2). Correspondingly, the Codex MRL for OTC is 0.2 g/g for Black Tiger Prawn (Penaeus monodon) (3), which is consistent with the value set for fish by the Japan Ministry of Health, Labor, and Welfare (4).
Table of Contents Feed for the Digital Edition of LCGC The Peak - April 2008 LCGC The Peak - April 2008 Contents LC3LC: Comprehensive Two-Dimensional Liquid Chromatography Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns Industry News Events Education LCGC The Peak - April 2008 LCGC The Peak - April 2008 - LCGC The Peak - April 2008 (Page 1) LCGC The Peak - April 2008 - LCGC The Peak - April 2008 (Page 2) LCGC The Peak - April 2008 - LCGC The Peak - April 2008 (Page 3) LCGC The Peak - April 2008 - Contents (Page 4) LCGC The Peak - April 2008 - Contents (Page 5) LCGC The Peak - April 2008 - Contents (Page 6) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 7) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 8) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 9) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 10) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 11) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 12) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 13) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 14) LCGC The Peak - April 2008 - LC3LC: Comprehensive Two-Dimensional Liquid Chromatography (Page 15) LCGC The Peak - April 2008 - Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination (Page 16) LCGC The Peak - April 2008 - Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination (Page 17) LCGC The Peak - April 2008 - Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination (Page 18) LCGC The Peak - April 2008 - Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination (Page 19) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 20) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 21) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 22) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 23) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 24) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 25) LCGC The Peak - April 2008 - A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns (Page 26) LCGC The Peak - April 2008 - Industry News (Page 27) LCGC The Peak - April 2008 - Events (Page 28) LCGC The Peak - April 2008 - Events (Page 29) LCGC The Peak - April 2008 - Education (Page 30) LCGC The Peak - April 2008 - Education (Page 31) LCGC The Peak - April 2008 - Education (Page 32)
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