LCGC The Peak - April 2008 - (Page 22)

22 THE PEAK APRIL 2008 Table I: Physical and chemical specifications of the C1 columnsa used Designation Subhead (A) Capcell PakC1 UG120 (S-5) (B) Capcell PakC1 UG120 (S-5) (C) Daisopak SP-200-3-C1-P (D) Daisopak SP-200-3-C1-P (E) Develoosil TMS-5 (F) Develoosil TMS-3 a 4.6 mm i.d. b No data Column length (mm) 35 50 35 50 50 50 dp (µm) 5 5 3 3 5 3 Surface area (m2/g) 300 300 300 300 300 300 Pore diameter (Å) 120 120 200 200 140 140 Pore volume (mL/g) Ndb Nd 1.1 1.1 1.15 1.15 Carbon content (%) 4 4 3 3 4.5 4.5 Experimental Procedure: Black Tiger Prawn tissues were minced fully and used as the blank samples. An accurately weighed 0.1 g sample was taken into a 1.5 mL microcentrifuge tube and homogenized with a handheld ultrasonic-homogenizer (model HOM100, 2 mm i.d. probe, Iwaki Glass Co. Ltd, Funabashi, Japan) for 30 s with 0.6 mL of a 20% (w/v) trichloroacetic acid (TCA) solution. After being homogenized, the capped tube was centrifuged at 12000 g for 5 min. The supernatant liquid was filtrated through the 0.45 m filter unit and the filtrate was then injected into an HPLC system. HPLC: The HPLC system included a model PU-980 pump and DG-980-50 degasser (Jasco Corp., Tokyo, Japan) equipped with a model CO-8010 column oven (Tosoh Corp., Tokyo, Japan), as well as a model SPD-M10A VP PDA detector (Shimadzu Scientific Instruments, Kyoto, Japan). Operating conditions: The analytical column was a Capcell Pak 70 C1 UG120 (S-5) packed with C1 methyl-silica, 5 m dp, 35 mm 4.6 mm i.d. (Shiseido Co. Inc., Tokyo, Japan) equipped with a guard column (5 mm 4.6 mm i.d.) containing the same packing material; the isocratic mobile phase was 0.1 mol/L citric acid; the flow-rate was 1.0 mL/min; column temperature was 45 °C; injection volume was 20 L; and the analytical time was less than 4 min. The PDA detector was operated at 200–380 nm. Monitoring the wavelength was adjusted to 363 nm, which represents an average maximum wavelength for all the target compounds.

Table of Contents Feed for the Digital Edition of LCGC The Peak - April 2008

LCGC The Peak - April 2008 Contents LC3LC: Comprehensive Two-Dimensional Liquid Chromatography Split Injections in Gas Chromatography: How to Reduce Inlet Discrimination A Simple and Solvent-Free Method for Determining Tetracyclines in Prawns Industry News Events Education

LCGC The Peak - April 2008

For optimal viewing of this digital publication, please enable JavaScript and then refresh the page. If you would like to try to load the digital publication without using Flash Player detection, please click here.