LCGC The Peak - July 2008 - (Page 18) 18 THE PEAK JULY 2008 Figure 10: SFC separation of A-F using (a) C18 and (b) cyano columns under SFC conditions (5% methanol in carbon dioxide, 1.3 mL/min flow rate, 1500 psi, 30 °C). Elution order of C18 column = E, D, C, A, G, B. Elution order of cyano column not determined. in SFC. The general behavior of an inverse elution order between isocratic reversedphase HPLC and SFC was observed. Lowering the pressure increased retention times of the tested compounds. This article has shown the benefit of SFC using reversed phase columns as an alternative technique to reversed-phase HPLC. References (1) K.G. Lynam and E.C. Nicolas, J. Pharm. Biomed. Anal. 11, 1197–1206 (1993). (2) L.M. Nogle, C.W. Mann, W.L. Watts Jr., and Y. Zhang, J. Pharm. Biomed. Anal. 40, 901–909 (2006). (3) K. Anton, C. Berger (Eds.), Supercritical Fluid Chromatography with Packed Columns: Techniques and Applications (Marcel Dekker Publishing, New York, 1998), pp. 19–58, 223–249. (4) W.H. Pirkle and C.J. Welch, J. Org. Chem. 49, 807–814 (1996). (5) M.S. Villeneuve and R.J. Anderegg, J. Chromatogr., A 826 217–225 (1998). (6) M. Alasandro. J. Pharm. Biomed. Anal. 14, 807–814 (1996). (7) K. Yaku, K. Aoe, N. Nishimura, and F. Morishita, J. Chromatogr., A 848, 337–345 (1999). (8) T.A. Berger and W.H. Wilson, J. Biochem. Biophys. Methods 43, 77–85 (2000). (9) J. Hochlowski, J. Olsen, J. Pan, D. Sauer, P. Searle, and T. Sowin, J. Liq. Chromatogr. Rel. Technol. 26(3), 333–354 (2003). (10) P.J. Edwards, Combinatorial Chem. High Throughput Screen 6, 11–27 (2003). (11) T.A. Berger and W.H. Wilson, J. Pharm. Sci. 83, 281–286 (1994). (12) T.A. Berger and W.H. Wilson, J. Pharm. Sci. 83, 287–290 (1994). (13) I.C. Bhoir, S.T. Patel, and M. Sundaresan, Talanta 48, 1179–1189 (1999). (14) W. Zou, T.L. Chester, and J.G. Dorsey, Anal. Chem. 72, 3620–3626 (2000). (15) J. Dolan, LCGC 21(4), 350–354 (2003). (16) Y. Patel, U.J. Dhorda, M. Sundaresan, and A.M. Bhagwat, Anal. Chem. Acta 362, 271–277 (1998).
Table of Contents Feed for the Digital Edition of LCGC The Peak - July 2008 LCGC The Peak - July 2008 Contents From the Editor Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference Industry News Events Education LCGC The Peak - July 2008 LCGC The Peak - July 2008 - LCGC The Peak - July 2008 (Page Cover1) LCGC The Peak - July 2008 - LCGC The Peak - July 2008 (Page Cover2) LCGC The Peak - July 2008 - LCGC The Peak - July 2008 (Page 3) LCGC The Peak - July 2008 - Contents (Page 4) LCGC The Peak - July 2008 - Contents (Page 5) LCGC The Peak - July 2008 - Contents (Page 6) LCGC The Peak - July 2008 - From the Editor (Page 7) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 8) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 9) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 10) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 11) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 12) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 13) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 14) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 15) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 16) LCGC The Peak - July 2008 - Evaluation of Nonpolar Reversed-Phase Columns Under Supercritical Conditions (Page 17) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 18) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 19) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 20) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 21) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 22) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 23) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 24) LCGC The Peak - July 2008 - Enhancing GC–MS Analysis of Trace Compounds Using a New Dynamic Approach to Reducing Background Interference (Page 25) LCGC The Peak - July 2008 - Industry News (Page 26) LCGC The Peak - July 2008 - Events (Page 27) LCGC The Peak - July 2008 - Events (Page 28) LCGC The Peak - July 2008 - Events (Page 29) LCGC The Peak - July 2008 - Education (Page 30) LCGC The Peak - July 2008 - Education (Page 31) LCGC The Peak - July 2008 - Education (Page 32) LCGC The Peak - July 2008 - Education (Page Cover4)
For optimal viewing of this digital publication, please enable JavaScript and then refresh the page. If you would like to try to load the digital publication without using Flash Player detection, please click here.