The Column - June 2009 - 12

The Column www.thecolumn.eu.com Tips & Tricks: GPC/SEC the most fundamental requirements in GPC/ SEC. Regarding the reliability of a GPC/SEC pump, four different cases (summarized in Table 3) can be discussed, that influence the result accuracy. The most dramatic deviation is observed for case 4. Molar mass deviations of more than 100% can be possible, just resulting from unwanted different flow-rates for calibration and sample evaluation. This can be easily avoided when working with an internal flow marker (or internal standard). For this approach, a monodisperse and low molar mass sample (the flow marker) is added to the solvent used to prepare the sample solutions. Based on the equation Vcorr. Vexp. VFlow marker exp. VFlow marker calib. References 1. T. Hofe and G. Reinhold, The Column, 3(12), 30–33 (2007). 2. L.H. Tung, J. Appl. Polym. Sci., 13, 775 (1969). 3. Harald Pasch; Bernd Trathnigg, HPLC of Polymers, Springer Verlag 1998, p.45ff. 4. Handbook of Size Exclusion Chromatography and Related Techniques (Editor Chi-san Wu); Second Edition, Marcel Dekker Inc. 2004; p.10. [3] the elution volumes will then be corrected by the ratio of the elution volume of the experiment and the elution volume of the flow marker during calibration. This easy concept enables a high reproducibility and keeps an existing calibration valid over a long time. This saves time and eluent. In addition the flow marker is a good sensor for unwanted changes of the column packing and unexpected changes in the analytical set-up. Thorsten Hofe studied chemistry in Mainz (Germany) and Toronto (Canada). He is the head of the PSS production department (columns, reference material and polymer synthesis). He is also involved in education and customer training. Daniela Held studied polymer chemistry in Mainz (Germany) and is working in the PSS software and instrument department. She is also responsible for education and customer training. Contact author: Thorsten Hofe E-mail: Thofe@polymer.de 12 1 9 Contents Tips & Tricks 2 13 Vosloo Rainville and Mather 6 17 News Meeting Review 8 20 Market Trends & Analysis Events & Contacts

The Column - June 2009

Table of Contents for the Digital Edition of The Column - June 2009

The Column - June 2009
Laboratory Equipment Qualification: Back to Basics
News
Market Trends and Analysis
Tips & Tricks: GPC/SEC
Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve ProductIon Confirmation
Meeting Review
Events
The Column - June 2009 - The Column - June 2009
The Column - June 2009 - Laboratory Equipment Qualification: Back to Basics
The Column - June 2009 - 3
The Column - June 2009 - 4
The Column - June 2009 - 5
The Column - June 2009 - News
The Column - June 2009 - 7
The Column - June 2009 - Market Trends and Analysis
The Column - June 2009 - Tips & Tricks: GPC/SEC
The Column - June 2009 - 10
The Column - June 2009 - 11
The Column - June 2009 - 12
The Column - June 2009 - Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve ProductIon Confirmation
The Column - June 2009 - 14
The Column - June 2009 - 15
The Column - June 2009 - 16
The Column - June 2009 - Meeting Review
The Column - June 2009 - 18
The Column - June 2009 - 19
The Column - June 2009 - Events
The Column - June 2009 - 21
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