The Column - June 2009 - 13

Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve Product Ion Confirmation Paul D. Rainville and Joanne Mather, Waters Corporation, Milford, Massachusetts, USA. Multiple peaks with the same MRM transition can be observed during method development or routine analysis in bioanalytical studies. These spurious peaks can be generated by chromatographic peak splitting, metabolites or matrix interferences. Product ion confirmation (PIC) functionality enables the confirmation of peak identity during MRM quantitative analysis by obtaining a quality product scan spectra during MRM MS analysis. The accurate quantification of candidate pharmaceuticals in bioanalysis relies on the use of sensitive, specific methodology. The advent of atmospheric pressure ionization sources in the late 1980s allowed the simple interfacing of liquid chromatography (LC) with tandem quadrupole mass spectrometry (MS). The selectivity and sensitivity provided by LC–MS–MS has made it the technique of choice for many bioanalytical studies. Bioanalytical scientists have relied on the specificity and sensitivity of the multiple reaction monitoring (MRM) MS acquisition mode to ensure that the correct analyte is quantified. However, even with the specificity of MRM analysis and modern sample preparation techniques, such as solid-phase extraction, extraneous peaks can interfere during sample analysis. The appearance of an extra peak during MRM analysis could be caused by chromatographic peak splitting, associated drug metabolites or matrix interferences. This occurrence of extra peaks during analysis requires additional experiments to be conducted to correctly identify the analyte of interest. With conventional tandem quadrupole mass spectrometers, these experiments require separate full scan MS and full scan MS–MS analyses to confirm the identity of the peaks in question. Many tandem MS instruments are unable to perform MRM and scan experiments simultaneously, in the timeframe of an LC peak, while maintaining data quality. Recently, Waters Corporation has demonstrated the use of novel MS technology that enables both MRM and full scan MS or product scan MS–MS to be acquired during a single injection. The company’s Xevo TQ mass spectrometer (see Figure 1) is equipped with a collision cell design wherein 13 1 9 Contents Tips & Tricks 2 13 Vosloo Rainville and Mather 6 17 News Meeting Review 8 20 Market Trends & Analysis Events & Contacts

The Column - June 2009

Table of Contents for the Digital Edition of The Column - June 2009

The Column - June 2009
Laboratory Equipment Qualification: Back to Basics
News
Market Trends and Analysis
Tips & Tricks: GPC/SEC
Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve ProductIon Confirmation
Meeting Review
Events
The Column - June 2009 - The Column - June 2009
The Column - June 2009 - Laboratory Equipment Qualification: Back to Basics
The Column - June 2009 - 3
The Column - June 2009 - 4
The Column - June 2009 - 5
The Column - June 2009 - News
The Column - June 2009 - 7
The Column - June 2009 - Market Trends and Analysis
The Column - June 2009 - Tips & Tricks: GPC/SEC
The Column - June 2009 - 10
The Column - June 2009 - 11
The Column - June 2009 - 12
The Column - June 2009 - Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve ProductIon Confirmation
The Column - June 2009 - 14
The Column - June 2009 - 15
The Column - June 2009 - 16
The Column - June 2009 - Meeting Review
The Column - June 2009 - 18
The Column - June 2009 - 19
The Column - June 2009 - Events
The Column - June 2009 - 21
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