The Column - June 2009 - 14

The Column www.thecolumn.eu.com Rainville and Mather the collision gas is always on, allowing both quantification (MRM) and characterization to be performed simultaneously on the peak as it elutes from the LC or ultra-performance liquid chromatography (UPLC) column, while maintaining good data quality. The new mode of operation, known as Waters ScanWave technology, allows ions within the collision cell to be accumulated and then separated according to their mass-to-charge (m/z) ratio. Synchronizing the release of these ions with the scanning of the second quadrupole mass analyser greatly improves duty cycle, which significantly enhances the signal intensity of full-scan spectra for both MS and product ions. Thus, as shown in the following work, the company’s tandem quadrupole MS functionality for product ion confirmation (PIC) enables the confirmation of peak identity during MRM quantitative analysis by obtaining a quality product scan spectra during MRM MS analysis. The LC system used for the experiment was an ACQUITY UPLC system combined with an ACQUITY UPLC BEH C18 (2.1 50 mm) column at a column temperature of 45 °C and a flow-rate of 500 L/min. A diluted ammonium hydroxide solution (0.1% NH4OH) was used for the first mobile phase and methanol for the second, with a gradient of 15–95%B/1 min. For the analysis, the company’s tandem quadrupole MS was used, with electrospray ionization set to positive ionization mode with capillary voltage of 3000 V and cone voltage of 30 V. The source temperature was set at 150 °C and the desolvation temperature at 600 °C, with a flow of 1000 L/h. Collision energy was 17 V and MRM transition m/z 501 293. Figure 1: The ACQUITY UPLC system with the Xevo TQ MS. Spiked Rat Plasma The experiment was conducted using rat plasma spiked with fluticasone propionate, a corticosteroid used for the treatment of asthma. The sample was prepared by solid-phase extraction using one of the company’s Oasis HLB Elution plate and analysed using UPLC–MS–MS. 14 1 9 Contents Tips & Tricks 2 13 Vosloo Rainville and Mather 6 17 News Meeting Review 8 20 Market Trends & Analysis Events & Contacts

The Column - June 2009

Table of Contents for the Digital Edition of The Column - June 2009

The Column - June 2009
Laboratory Equipment Qualification: Back to Basics
News
Market Trends and Analysis
Tips & Tricks: GPC/SEC
Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve ProductIon Confirmation
Meeting Review
Events
The Column - June 2009 - The Column - June 2009
The Column - June 2009 - Laboratory Equipment Qualification: Back to Basics
The Column - June 2009 - 3
The Column - June 2009 - 4
The Column - June 2009 - 5
The Column - June 2009 - News
The Column - June 2009 - 7
The Column - June 2009 - Market Trends and Analysis
The Column - June 2009 - Tips & Tricks: GPC/SEC
The Column - June 2009 - 10
The Column - June 2009 - 11
The Column - June 2009 - 12
The Column - June 2009 - Confirming Peak Identification in Bioanalytical Studies Using Dual-Scanning MS Technology to Achieve ProductIon Confirmation
The Column - June 2009 - 14
The Column - June 2009 - 15
The Column - June 2009 - 16
The Column - June 2009 - Meeting Review
The Column - June 2009 - 18
The Column - June 2009 - 19
The Column - June 2009 - Events
The Column - June 2009 - 21
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