The Column - September 2006 - (Page band1)

completely new approach. Here the mobile phase remains constant and optimization of the stationary phase is the primary goal. POPLCTM is based on the theory of the Prisma model that has been used before to optimize mobile phases mainly in thin layer www.poplc.de chromatography TLC and normal phase HPLC [1]. The Prisma is shown in Fig. 1. Prisma Model A New and Effective Tool to Optimize HPLC Methods! Introduction Optimization of Liquid Chromatography Separations computer software is used to optimize the mobile has been done with several techniques in the past. phase and therefore the separation. Those Some scientists prefer to keep the stationary phase software support is very helpful in some cases and constant and do the optimization mainly by changing saves a lot of time of trial and error in comparison to pH, type of organic modifier methanol or acetonitrile manual procedures. Never the less it will not help to or the temperature of the mobile phase. Others first resolve two components if those can not be apply a standard gradient on several different types of separated on the stationary phase which is used for The vertical sides of the prism correspond to the stationary phases and use the best suited column for the corresponding separation. retention strength of a given stationary phase with further optimization of mobile phase. There are also In Phase OPtimized Liquid Chromatography regard to a given analyte. The optimal retention some optimization procedures existing where POPLCTM the optimization procedure follows a strength for this analyte is somewhere on the surface that is built by the upper irregular triangle of the prism. http://www.poplc.de http://www.poplc.de

Table of Contents for the Digital Edition of The Column - September 2006

Contents
News
Opinion
Market Trends & Analysis
Country Focus
Determination of Chloramphenicol in Food using Automated Solid-Phase Extraction and LC-MS
Q&A
Supplies & Services

The Column - September 2006

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