The Column - October 2008 - (Page 12)

News The Column www.thecolumn.eu.com October 2008 News Delicate ecosystems exist on many oceanic islands across the globe. Plant species in particular are often threatened by invasive species, such as the presence of rats. This has been the case on the 10-acre island of Mokapu, located 1 km off the north coast of Molokai in Hawaii. It is an Hawaiian State Seabird Sanctuary and supports a diverse native coastal plant community. The US Fish and Wildlife Service designated Mokapu as critical habitat for the endangered Peucedanum sanwichense, a large herbaceous plant together with native lama and palm trees such as Diospyros spp., and Pritchardii hillebrandii. The US government believed it probable that the presence of rats was responsible for the severe decline in this native flora. Toxic pellets were deployed that contained the active ingredient, diphacinome at 50 ppm to exterminate the rodent population. Non-target species such as fish tissues and limpets were then monitored for any adverse effects or sub-lethal doses. Extraction of the diphacinone from the matrix of interest (sea water, fillet, whole limpet and bait) was conducted and subsequent concentration and purification of the extract by solid-phase extraction (SPE) or low performance size-exclusion chromatography (LP-SEC). HPLC was used for the analytical separation of diphacinone followed by ultraviolet-visible photodiode array absorbance (PDA) detection and quantification methods. Coumarin was used as the instrumental internal standard. For quantification of diphacinone the team used a Surveyor HPLC system comprising an autosampler, gradient pump, PDA detector and XCalibur chromatography data collection and processing software. A Luna C18(2) 100 Å, 150 2 mm 3 µm analytical column with a SecurityGuard C18 guard column cartridge was used for the separation of diphacinone. The mobile Martin Harvey/Getty Images Eradicating invasive species but at a cost? phase used the ion-repair reagent 0.03 M TBAH-IP in water adjusted to a pH of 6.0 using 2 N o-phosphoric acid. Methanol/0.03 M TBAH-IP was used for the mobile phase and delivered isocratically. The sample was then applied to the column via 20 µL full-loop injections. Detection of the diphacinone was by wavelength scanning from 200–400 nanometres. The sample preparation methods increased in their complexity as the complexity of the sample matrices increased (i.e., sea water fillet limpet). Preparation of bait composite samples required strong and specific reaction conditions to completely release diphacinone bound in the bait matrix and the wax-like coating materials. The efficiency and selectivity of the preparatory methods that were developed for the study was reflected in the achievement of consistently low detection limits without background interferences for all matrices. The report reached the conclusion that no matrices contained diphacinone at or above the method limit of detection, which ranges from around 10 to 18 parts per billion. The researchers found that “The isolation, concentration and high-performance liquid chromatographyphotodiode array method performed well throughout the analyses of all sample matrices. No instances of interfering compounds co-eluting with diphacinone (or with coumarin) were noted. The co-elution of compounds that interfere with analyte response (quantification) or analyte spectra (identification) typically results in reporting an analyte as not quantifiable at an increased limit of quantification.” The full report and methodology can be downloaded at http://pubs.usgs.gov/of/2008/1285/pdf/OFR2008-1285.pdf Chromatide to form new scientific advisory board Polymer technology firm, Chromatide has formed a highly experienced scientific advisory board (SAB) to help grow its intellectual property (IP) portfolio. The SAB will comprise Dr Brian Adger, Dr Eric Atherton and Professor Link: 12 Nigel Slater who between them represent over 25 years of extensive knowledge and experience in the pharma, chemical, peptide chemistry and bioproduct manufacture industries. Clare Hildred, chief executive officer of Chromatide said, “The combination of skills and expertise Dr Adger, Dr Atherton and Professor Slater bring will help us to continue to drive the company business forward by providing highly regarded, objective views of the IP we are developing.” Visit www.chromatide.com www.chromatide.com http://www.thecolumn.eu.com http://pubs.usgs.gov/of/2008/1285/pdf/OFR2008-1285.pdf http://www.chromatide.com http://www.chromatide.com

Table of Contents for the Digital Edition of The Column - October 2008

The Column - October 2008
Contents
Rapid Fatty Acid Fingerprinting in Microalgae by Gas Chromatography: Analytical Opportunities
News
Incognito: Total Solutions — Really?
Market Trends and Analysis: Market Profile: The Analytical and Life Science Instruments Industry
Tips and Tricks: GPC/SEC: Understanding Positive and Negative Detector Signals
Biodiesel Patterns Reflect Quality: Analysis According to DIN EN 14103
Bringing Together GC and LC on an MS–TOF System
Q&A

The Column - October 2008

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