The Column - November 2007 - (Page 28)

Widstrand et al. The Column November 2007 Highly Selective Trace Level Extraction using Molecularly Imprinted Polymer Solid-Phase Extraction Christine Widstrand,1 Brian Boyd,1 Olga Shimelis,2 An Trinh2 and Hillel Brandes,2 1MIP Technologies, Lund, Sweden, 2Supelco, Bellefonte, Pennsylvania, USA. This article describes a highly selective method to extract trace levels of chloramphenicol using molecularly imprinted polymer solid-phase extraction (MIP SPE). Chloramphenicol (CAP) is a broad spectrum antibiotic that has recently been determined as a probable causative agent of aplastic anaemia and a possible carcinogen in humans. As a result of these health concerns, the EU, the US and Canada have banned the use of CAP in food-producing animals and livestock1. However, because the drug is still widely available in developing countries and no “safe” residue levels can be defined in food, public health concerns still arise. Current guidelines have fixed a “zero tolerance” level for this antibiotic. A minimum required performance limit (MRPL) for CAP determination was recently set by the EU at 0.3 µg/kg in all foods of animal origin.2 It is, therefore, critical to develop both a selective and sufficiently sensitive analytical assay to control and monitor CAP residues in such difficult food matrices. Existing methods for the determination of CAP include immunoassays,3 microbiological methods,4 sensors5 and chromatographic methods using gas chromatography–mass spectrometry (GC–MS)6 and liquid chromatography–mass spectroscopy–mass spectroscopy (LC–MS–MS).7 The major challenge for trace-level determination of analytes in complex matrices, such as food, is the sample preparation step, which can become the bottleneck for high sample throughput Author: Christine Widstrand E-mail: 28

Table of Contents for the Digital Edition of The Column - November 2007

The Column - November 2007
Food for Thought Q&A
Market Trends and Analysis
Meeting Review: 12th Desty Memorial Lecture
Separation and Purification of Chiral Compounds by Two-Dimensional Supercritical Fluid Chromatography
Highly Selective Trace Level Extraction using Molecularly Imprinted Polymer Solid-Phase Extraction

The Column - November 2007