Phenomenex Catalog International 2009 - (Page 14) Strata SPE Method Development ® 3 Simple Steps to Choosing an SPE Product 1. What is the Analyte? Identify the most specific Strata sorbent by matching the analyte functional group to the sorbent functional group. Selectivity & High Retention Strength (From High to Low) Analyte Moiety Analyte Functional Group SPE Mechanism Sorbent Functional Group Sorbent Recommended Strata Sorbent(s) SAMPLE PREPARATION/SPE Polar/Non-polar Bases Basic Basic Acidic Acidic Hydrocarbon Aromatic Hydroxyl Amino Neutral /R / polar groups RNH3+ Weak Base NR4+ Strong Base RCO2- Weak Acid RCO3- Strong Acid R R - OH R - NH2 Neutral Polar/Non-polar Ion Exchange (Cation) Ion Exchange (Cation) Ion Exchange (Anion) Ion Exchange (Anion) Reversed Phase Reversed Phase Normal Phase Normal Phase Strong Cation Exchange Weak Anion Exchange / polar groups O3S – strong O2C – weak + R3N – strong + H3N – weak / CN OH O3S – strong + H3N – weak Reversed Phase Dipole-Dipole/Aromatic Strong Cation Weak Cation Strong Anion Weak Anion Hydrocarbon Aromatic Polar Polar X, XL X-C, SCX, XL-C X-CW, WCX SAX, X-AW X-AW, NH2 C18-E, C18-U, C18-T, C8 X, PH, SDB-L, XL CN, NH2 Si-1, CN ABW STRATA SPE METHOD DEVELOPMENT | 2. What is the Matrix? Identify the possible SPE retention mechanism: Reversed Phase (RP), Ion-Exchange (IEX) or Normal Phase (NP): The sample solvent composition will guide you towards an appropriate SPE mechanism. Aqueous Water, Urine, Plasma, Blood, Aqueous Tissue Prep. Organic Water miscible, Methanol Acetonitrile, Acetone Add H20 Add Hexane Organic Not water miscible Hexane Dichloromethane For ionic species only Reversed Phase Strata-X, XL, C18-E, C8, C18-U, PH or Ion-Exchange Strata-X-C, X-CW, XL-C, X-AW, SAX, NH2, ABW, Screen-C, Screen-C GF, SCX, WCX, Screen-A Ion-Exchange Strata-X-C, X-CW, XL-C, X-AW, SAX, NH2, ABW, SCX, WCX Normal Phase Strata NH2, CN, Si-1, FL-PR, EPH, Alumina-N, Eco-Screen 3. What is the Volume? To select the proper sorbent mass, it is first necessary to determine the volume of sample needed to be extracted in order to meet method detection limits (not including buffer). Two tables are included below: Polymer-based and silica-based. This is necessary because the large surface area of polymeric sorbents, such as Strata-X, have a higher analyte capacity per gram than silica-based sorbents. Suggested Loading Capacity Table 1 Polymer-Based Sorbents (Strata-X, X-C, X-AW, X-CW, XL, XL-C, and SDB-L) Sample Matrix Blood, serum, plasma Urine Filtered tissue homogenates Table 2 Silica-Based Sorbents (Strata C18-E, C8, SCX, SAX, WCX, NH2, etc.) Sample Matrix Blood, serum, plasma Urine Filtered tissue homogenates Sorbent Mass 30 mg sorbent per 250 µL 30 mg sorbent per 1 mL 60 mg sorbent per 100 mg tissue Sorbent Mass 50 mg sorbent per 250 µL 50 mg sorbent per 500 µL 100 mg sorbent per 100 mg tissue Environmental Samples Water (particulate-free) drinking Water (particulate-laden) rivers, runoff, etc. Soil Extracts Sorbent Mass 200 mg/100 mL – 400 mL sample 500 mg/100 mL – 400 mL sample 500 mg/100 g of soil extract Environmental Samples Water (particulate-free) drinking Water (particulate-laden) rivers, runoff, etc. Soil Extracts Sorbent Mass 500 mg/100 mL - 500 mL sample 1 g/100 mL - 500 mL sample 1 g/100 g of soil extract 14 Phenomenex http://www.phenomenex.com/WorkArea/showcontent.aspx?id=8409
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