Phenomenex Catalog International 2009 - (Page 28) Strata -X Polymeric (SPE) Sorbents ™ Strata-X-AW: Extract Acids by Mixed-Mode Weak Anion Exchange • Cleaner extracts • Weak anion exchange mechanism allows for elution at high and low pH SAMPLE PREPARATION/SPE • Elution with LC/MS compatible buffer eliminates the dry down step resulting in faster analysis Selective Elution of Acidic Analytes The weak anion exchange functionality of Strata-X-AW allows for cleanup of strong acids such as sulfonates that are irreversibly retained on strong anion exchangers. The pKa of the Strata-X-AW sorbent is more versatile in comparison to strong anion exchangers, giving a wider range of flexibility. Also, Strata-X-AW sorbent allows for the selective elution of acidic compounds under acidic conditions or basic conditions for direct inject analysis onto a MS. If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE! Material Characteristics Particle Size (µm) Pore Size (Å) Surface Area (m2/g) pH Stability Ionic Capacity pKa 33 85 800 1-14 0.60 meq/g ~9 | STRATA-X POLyMERIC (SPE) SORBENTS Weak Anion Exchange & Reversed Phase Ordering Information Sorbent Format Mass Tube 30 mg 60 mg 200 mg 500 mg 100 mg 200 mg 500 mg Giga™ Tube 500 mg 1g 96-Well Plate 10 mg 30 mg 60 mg ) n NH NH2 Part Number 8B-S038-TAK 8B-S038-UBJ 8B-S038-FBJ 8B-S038-HBJ 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH 8B-S038-HDG 8B-S038-JDG 8E-S038-AGB 8E-S038-TGB 8E-S038-UGB Unit 1 mL (100/box) 3 mL (50/box) 3 mL (50/box) 3 mL (50/box) 6 mL (30/box) 6 mL (30/box) 6 mL (30/box) 12 mL (20/box) 12 mL (20/box) 2 Plates/Box 2 Plates/Box 2 Plates/Box Price Elute 2x with 5 % NH4OH in Methanol ) Condition with Methanol Equilibrate with Water Load Diluted sample pH within 6-8 Wash with 25 mM Ammonium Acetate Wash with Methanol Strata-X-AW General Method Elute 2x with 2 % Formic Acid in Methanol The above is a convenient starting point for SPE method development. Further optimization may be required to tailor the method to your specific needs. 28 Phenomenex
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