Phenomenex Catalog International 2009 - (Page 31) High-Throughput Sample Preparation On-Line SPE Format • Simultaneous concentration and cleanup of analytes • Effective removal of matrix components • Effectively remove protein contaminants, salts and ions from samples in one step • Cleans up and concentrates target analytes in minutes with one sample injection • Compatible with standard HPLC systems for hundreds of injections without changing cartridges Phase Strata C18 Strata C8 Strata-X Strata-X-CW FREE White Paper on Rapid Extraction and MS Quantitation of Target Analytes from Biological Sample Matrices SAMPLE PREPARATION/SPE Part No.: 4431_U Description Silica-based trifunctional C18 Silica-based trifunctional C8 with hydrophobic endcapping of silanols Polymeric sorbent with strong pi-pi retention mechanism Weak cation exchange mechanism allows for elution at high and low pH Recommended Application Non-polar analytes Moderately polar analytes Polar analytes Basic analytes | Simple On-line SPE Setup Sample Sample Pump 1 Pump 1 Pump 1 Pump 1 Method for General Reversed Phase Screening Refer to schematic at left A: 0.1 % formic acid/water Time Pump 1 (Load) 95 % A: 5 % B B: 0.1 % formic acid/acetonitrile Valve Load position Switch from Load to Elute position Switch from Load to Elute position Load position Pump 2 (Elute) 95 % A: 5 % B 95 % A: 5 % B 15 % A: 85 % B 95 % A: 5 % B 95 % A: 5 % B Elution Position Elution Position HIGH-THROUGHPUT SAMPLE PREPARATION Load Position Load Position Waste Waste SPE SPE Waste Waste SPE SPE 0 0.5 2.8 3.0 Pump 2 Pump 2 Mass Mass Spectrometer Spectrometer Column Column Pump 2 Pump 2 Mass Mass Spectrometer Spectrometer Column Column Ordering Information Strata On-Line Extraction Cartridge Part No. Description 00M-S033-B0-CB Strata-X on-line extraction cartridge, 20 x 2.0 mm 00M-S036-B0-CB Strata-X-CW on-line extraction cartridge, 20 x 2.0 mm 00M-S039-B0-CB Strata C18 on-line extraction cartridge, 20 x 2.0 mm 00M-S101-B0-CB Strata C8 on-line extraction cartridge, 20 x 2.0 mm CH0-5845 Cartridge holder, 20 mm Unit/Box ea ea ea ea ea Price For Fluid Processors, see p. 342 Strata-X Method Development Kits Two techniques to choose from • Sample cleanup and concentration • High-throughput screening Ordering Information Sample Preparation Method Development Kits Part No. Description Unit Strata-X Method Development Kit for Sample Cleanup and Concentration ea KS0-7908 Strata-X 200 mg/3 mL tubes (5 ea) Strata-X-C 200 mg/3 mL tubes (5 ea) Strata-X-CW 200 mg/3 mL tubes (5 ea) Strata-X-AW 200 mg/3 mL tubes (5 ea) Strata Method Development Demo Software Strata-X Method Development 96-Well Plate Kit for High-Throughput Screening ea KS0-8241 Strata-X 10 mg/well (3 rows) Strata-X-C 10 mg/well (3 rows) Strata-X-CW 10 mg/well (3 rows) Strata-X-AW 10 mg/well (3 rows) KS0-8209 Strata-X 30 mg/well (3 rows) Strata-X-C 30 mg/well (3 rows) Strata-X-CW 30 mg/well (3 rows) Strata-X-AW 30 mg/well (3 rows) ea For Strata Starter Kit for Protein Precipitation, see p. 30 Price Phenomenex 31 http://www.phenomenex.com/lib/4431.indd.pdf
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