Phenomenex Catalog International 2009 - (Page 32) SPE Vacuum Manifolds Vacuum Manifolds for Processing Samples • Multiple sample processing saves time and money • Process up to 12 or 24 samples at one time • Process up to 10 large volume samples at one time • Female Luer inlets fit all male Luer tipped SPE tubes and cartridges • Inert Teflon®, polypropylene, and stainless steel accessories available for flow control • Glass chamber allows easy visual monitoring • Adjustable racks accommodate a variety of test tubes, vials and flasks Stopcock Glass Chamber Collection Rack Vacuum Gauge Valve Assembly SAMPLE PREPARATION/SPE | Sample Processing Guidelines for Strata-X Sorbents Vacuum Manifolds: • Maximize throughput with simple, cost effective batch processing of up to 96 samples per hour. (24 samples per 15 minutes) • Fits 13 mm and 16 mm test tubes up to 125 mm in height. • “Slow is Safe” for loading and elution. A flow rate: 1 – 3 drops per second (1 – 3 mL /min) is recommended doing the loading and elution steps for typical small volume samples ( 100 mL) in large cartridges (>1 gram) may be processed at flow rates between 5 – 10 mL/minute. • Flow rate is easily adjusted via master vacuum controller or individual stopcocks if necessary. SPE VACUUM MANIFOLDS • Individual stopcocks are typically not needed when using the strata-X family of sorbents (Strata-X, X-C, X-CW, X-AW, XL, XL-C). They are very forgiving of improper flow rates and are truly resistant to deconditioning effects caused by excessive drying during the method. • Reversed phase methods are more forgiving of fast flow rates than ion-exchange or normal phase. • Conditioning and Wash steps are generally not flow critical. Processing Large Sample Volumes Reservoir/Empty Syringe Barrel TEFLON OR VACUUM RESISTANT TUBING � ADAPTOR CAP � Have Large Sample Volume but Need a Small Bed Mass? Use an adaptor cap to attach another SPE tube, which can be used to increase the reservoir size for washing or eluting solvents. Sample Vacuum Manifold Waste Trap Vacuum Pump Description ❶ Teflon or Vacuum Resistant Tubing (1/8 inch O.D.) ❷ Adaptor Cap Part No. AT0-2956 AH0-7191 Adaptor Cap (AH0-7191) 32 Phenomenex
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