Phenomenex Catalog International 2009 - (Page 4) Filtration Syringe Filters Phenex FILTRATION ™ best seller For sample and solvent filtration prior to chromatography • Rapid filtration of HPLC and GC samples prior to analysis • Particulate, PVC, and extractable-free filters • Less system down time • More consistent, reproducible results • Increased column lifetime Phenex Offers: • • • • • Maximum chemical compatibility Minimum extractables Excellent flow rate High total throughput Certified quality • • • • 100 % integrity tested Low hold-up volume Low protein adsorption Bi-directional use SyRINGE FILTERS | Syringe Filter Selection Guide 1. Choose filter diameter based on sample volume 4 mm Diameter ≤ 2 mL Sample Volume 15 - 17 mm Diameter 2 - 10 mL Sample Volume 25 - 28 mm Diameter 10 - 100 mL Sample Volume 2. Choose a pore size based on the nature of your sample and chromatographic method Sample Description General aqueous or mixed organic samples prior to HPLC analysis with column packed with > 3 µm particles. General clarification of GC, SFC, CE, and GPC samples. Viscous samples or samples containing high levels of particulate matter. General aqueous or mixed organic samples prior to HPLC analysis with columns packed with < 3 µm particles. Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples. Gas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods. Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such as some environmental or food and beverage applications. In-line Glass Fiber Pre-Filter or Integrated Glass Fiber Filter Unit Recommended Filter Pore Size 0.45 µm 0.20 µm 3. Choose a filter membrane according to the characteristics of your sample and filtering objective Membrane Type RC (Regenerated Cellulose) Recommended Uses Hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions, making them one of the most universal filter materials used prior to chromatography. Phenex-RC filters also exhibit fast-flow and ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, Phenex-RC membranes are broadly recommended as an excellent general purpose/high-performance sample filter for most applications. PTFE is an inherently hydrophobic membrane excellent for filtration of organic-based, highly acidic or basic samples and solvents. Widely used in chromatography, it is especially well suited for the clarification of non-aqueous samples. Although this membrane is hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water. Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many life science clarification applications. Phenex-PES membranes typically offer better chemical resistance than cellulose acetate and are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers. Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic samples. For applications that require low protein or non-specific binding characteristics, Phenomenex recommends Phenex-RC (Regenerated Cellulose) filters. Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In combination with a glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological sample filtration and clarification. Phenex-GF (Glass Fiber) filters are made of inert borosilicate glass and have a nominal 1.2 µm pore size. They are commonly used with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples, soil samples, fermentation broth samples, removal of yeasts, molds, etc.). Glass Fiber filters can be used alone or in series with other Phenex filter membranes such as the 0.45 µm pore Phenex-RC filter to reduce clogging of the membrane and optimize flow. PTFE, Teflon® (Polytetrafluoroethylene) PES (Polyethersulfone) NY (Nylon) CA (Cellulose Acetate) GF (Glass Fiber) Phenex is a trademark of Phenomenex, Inc. 4 Phenomenex http://www.phenomenex.com/products/brands/view.aspx?id=11519
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