1223 OB 4.4 MID 43.9 BS 211 CO 3.3 MID 4.4 BS 3.3 GluCer d18:1/24:1 MID 475 4L/PS-NA Control CB 3.1 Fold change 4L/PS-NA Control CB 292 Fold change 4L/PS-NA Control BS CB 23.5 32.6 Fold change GluCer d18:1/22:0 CO 5.8 OB CO 10.8 13.0 OB 6.7 GluCer d18:1/18:0 0 100 200 300 400 0 2 4 6 8 0 5 10 15 20 25 OB 6.0 OB 8.4 OB 10.9 MID 27.8 MID 444 BS 321 CO 4.7 MID 12.6 BS 6.4 Total GluCer CO 3.7 4L/PS-NA Control CB 11.4 Fold change 4L/PS-NA Control CB 362 Fold change 4L/PS-NA Control BS CB 34.1 17.1 Fold change GluCer d18:1/24:0 CO 4.5 GluCer d18:1/20:0 Glucosylceramide 0 500 1000 1500 2000 2500 0 20 40 60 80 100 0 50 100 150 200 250 B OB 0.7 OB 0.9 OB 0.9 MID 2.2 BS 1.2 MID 1.5 BS 0.9 CO 1.1 MID 1.8 BS 0.9 GalCer d18:1/24:1 CO 1.1 GalCer d18:1/22:0 CO 1.5 CB 0.6 Fold change 4L/PS-NA Control CB 0.6 Fold change 4L/PS-NA Control CB 0.8 Fold change 4L/PS-NA Control 0 1000 2000 3000 0 100 200 300 0 20 40 60 OB 0.7 OB 0.9 OB 0.8 Galactosylceramide GalCer d18:1/18:0 MID 1.7 BS 1.0 MID 1.2 BS 0.9 CO 1.2 MID 1.7 BS 0.9 Total GalCer CO 1.1 GalCer d18:1/24:0 CO 1.3 GalCer d18:1/20:0 CB 0.6 Fold change 4L/PS-NA Control CB 0.6 Fold change 4L/PS-NA Control CB 0.7 Fold change 4L/PS-NA Control Figure 3. LC-ESI-MS/MS explicates that HexCer elevations are attributed to GluCers. (A) Quantitative levels of total GluCers and GluCer species in 4L/PS-NA brains compared with the control. (B) Quantitative levels of total GalCers and GalCer species in 4L/PS-NA brains vs. control. Fold change of lipids levels in 4L/PS-NA over the control are shown under each bar. n = 2 mice. 0 10 20 30 0 2 4 6 8 0 50 100 150 200 250 A pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue pmole/mg tissue