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Reproductive Sciences Vol. 25, Supplement 1, March 2018

27 of 171(15.8%);(p=0.007)), however after adjusting for gestational
diabetes, age and weight, no significant difference was found between
the two groups (p=0.127).
CONCLUSION: The change in ACOG's new recommendation on timing
of delivery for patients with gestational hypertension does not increase the
rate of cesarean delivery. This information is important for counseling.
*Figure(s) will be available online.

F-183
Impaired Cognitive Function in Experimental Preeclampsia. Abbie
Johnson†, Amanda Whitbeck, Justin Miller, Marilyn Cipolla*. University
of Vermont, Burlington, VT, United States.
INTRODUCTION: Formerly preeclamptic women report cognitive
impairment and have greater incidence of cerebral white matter lesions
as early as ~ 5 years after the index pregnancy. The strong association
between white matter disease and cognitive impairment is well established;
however, whether cognitive impairment is present during preeclampsia
(PE) remains unknown. We hypothesized that rats with experimental PE
would have poor cognitive function compared to the normal pregnant
state. Here, we investigated cognitive function in a rat model of PE using
established behavioral tasks to assess learning and memory.
METHODS: Sprague Dawley rats that were either late-pregnant (LP) or
with experimental PE (ePE) were used (n=12/group). ePE was induced
by maintaining rats on a high cholesterol diet d7-21 of gestation. Morris
water maze (MWM) was used to test hippocampal-dependent short-term
memory, and a novel object recognition task (NOR) to test corticalhippocampal-dependent recall and working memory. MWM consisted
of four timed-swim trials on three consecutive days (d18-20) in which
latency to a submerged platform was determined for each trial. On day
four (d21), rats had a single swim test with the platform removed and the
latency to swim to the platform zone measured. The NOR task consisted
of two 120 sec object trials with a two-hour inter-trial interval (d21). The
first trial consisted of rats exploring two identical objects. In the second
trial, one object was replaced with a novel object and the amount of time
spent exploring the novel and familiar objects quantified.
RESULTS: Rats with ePE had longer latencies to find the platform
compared to LP rats (Figure 1), indicating impaired short-term memory
in ePE.
*Figure(s) will be available online.
Further, ePE rats took longer to reach the platform zone than LP rats (41
± 12 sec vs. 15 ± 4 sec; p<0.05) that was not due to differences in swim
speed. As expected in the NOR, LP rats spent the majority (60 ± 8 %) of
time exploring the novel object. However, ePE rats only spent 45 ± 6 %
of the time investigating the novel object, and the majority of the time
exploring the familiar object, suggesting impaired recall and recognition.
CONCLUSION: Impaired cognitive function was present in ePE rats.
Both hippocampal- and cortical-hippocampal-dependent learning and
memory processes were impaired, suggesting ePE affected multiple brain
regions involved in cognition. Overall, understanding how PE effects
cognitive function during the index pregnancy adds to our understanding
of the pathological processes occurring in PE that may contribute to
progressive cognitive impairment later in life.

F-184
The Performance of the Urinary Congo Red Retention Assay
Prepregnancy and Considerably Prior to Pre-Eclampsia in a
Longitudinal Pregnancy Cohort. Rebecca E Rieck†,4 Kara Rood,2
Catalin S Buhimschi,1 Carole A McBride,4 Irina A Buhimschi,1,3 Ira
Bernstein*.4 1The Ohio State University College of Medicine, Columbus,
OH, United States; 2The Ohio State Unviersity College of Medicine,
Columbus, OH, United States; 3The Research Institute at Nationwide
Children's Hospital, Columbus, OH, United States; 4University of Vermont
Medical Center, Burlington, VT, United States.
INTRODUCTION: The Congo Red Dot test (CRD) is a urine pointof-care diagnostic test for pre-eclampsia (PE) by detecting misfolded
proteins. We sought to evaluate the presence and natural history of
congophilia in a longitudinal cohort of pregnant subjects, followed from
pre-conception to weeks before onset of clinical signs/symptoms of PE.

Scientific Abstracts

METHODS: Urine samples from 124 patients were collected in a
prospective clinical study (at the time of first visit mean age: 30.2 years;
mean pre-pregnancy BMI 25.8 kg/m2). 79 patients were nulliparous and
43 patients had history of PE in prior pregnancy. The study consisted
of visits at three time points: prior to pregnancy, 12-14 weeks, and
29-32 weeks. 24 hour urine protein collections, blood pressures and
serum creatinine were assessed at each visit. Pre-pregnancy baseline
results include mean 24hr proteinuria of 174 mg + 103 and mean serum
creatinine clearance: 128 ml/min + 32. 23 patients had pathologic prepregnancy proteinuria (> 300 mg/24 hours). Of all the patients, 100 went
on to pregnancy. 18 patients developed gestational hypertension and 19
patients developed PE. 8 patients went on to develop preterm PE with
severe features; 7 of these patients had PE in their previous pregnancy.
The mean time between urine collection and the diagnosis of PE with
severe features was 34 ± 8 days with a minimum time of 20 days. We
performed the Congo Red Dot test (CRD) using a mixture of aggregated
proteins to serve as a positive control.
RESULTS: None of the prepregnancy samples, including patients with
pathologic (> 300 mg/24 hours) proteinuria displayed congophilia.
Furthermore, none of the 3rd trimester samples tested positive at the
asymptomatic stage of the disease. Positive controls samples run with
each assay displayed congophilia with a coefficient of variation of 14.2%.
CONCLUSION: Women with history of prior preterm PE and
pathologic proteinuria do not display congophilia, implying that, the
misfolded proteins that cause urinary CR retention at the time of clinical
manifestation of the disease are cleared following pregnancy.

F-185
Placental Density and Morphology in Preeclampsia. Ryan Schlueter†,
Ingrid Chern, Hyeong Jun Ahn, Nicole Kurata, Dena Towner*. University
of Hawaii, Honolulu, HI, United States.
INTRODUCTION: The aim of this study is to compare placental
density with mathematical modeling and morphologic characters in term
preeclampsia and control pregnancies.
METHODS: The study is a retrospective chart review of women
delivering at Kapiolani Medical Center for Women and Children from
February, 2011 through February, 2016. Charts were abstracted for case
patients with ICD-9 diagnosis of preeclampsia and normal deliveries were
used as a control. Only subjects with a pathology report were included.
Maternal and fetal demographics were recorded. Placental measurements
of weight (g), length (L), width (W), and height (H) as well as histologic
characteristics were documented. Volume (cm3) of the placenta was
assessed by using a cylinder where v = πr2h, mean r was calculated from
placental dimensions to assume circular shape of placental disc. The
density was calculated in (g/cm3). Continuous variables were compared
with t-tests and Wilcoxon rank sum. Categorical variables were compared
with Chi-square tests at 95% CI.
RESULTS: During the study period a total of 306 controls and 139 women
with preeclampsia were identified. No significant difference in age existed
between the groups (28 vs 28 years) (p=0.6243). In regards to gravidity
women in the preeclampsia group reflected 2.3 versus control of 3.0
(p<0.0001), parity of preeclampsia 1.8 versus control 2.3 (p<0.0001) and
gestational age in preeclampsia 38.2 weeks versus control of 38.7 weeks
(p<0.0001). The birth weight in the control group was 7.1 pounds and in
the preeclampsia population was 6.7 pounds (p=0.0002). There was no
significant difference in the APGAR score or cord gases. Placental weight
was 475.5 g in the control group and 474.5 g in the preeclampsia group
(p=0.93). The volume in preeclampsia placentas was 792.9 cm3 and 855.6
cm3 in the control group, so while less was not statistically significant
(p=0.06). Density was calculated as 0.62 g/cm3 in preeclampsia and 0.57
g/cm3in the control group which was significantly different (p=0.03).
When stratified for severe preeclampsia the density in the control group
was still 0.57 g/cm3 versus 0.62 g/cm3 in severe preeclampsia which was
significant (p=0.03).
CONCLUSION: Our study demonstrates that women with preeclampsia
tend to have decreased gravidity, parity and neonatal birth weight.
Placentas from mothers with preeclampsia are smaller in volume and
appear to be denser. The results support that preeclampsia has an origin



Table of Contents for the Digital Edition of SRI Supplement to Reproductive Sciences - Volume 25 Number 1 - March 2018

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SRI Supplement to Reproductive Sciences - Volume 25 Number 1 - March 2018 - Cover3
SRI Supplement to Reproductive Sciences - Volume 25 Number 1 - March 2018 - Cover4
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_december2020
https://www.nxtbook.com/nxtbooks/sage/psychologicalscience_demo
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_october2020
https://www.nxtbook.com/nxtbooks/sage/fai_202009
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_august2020
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_june2020
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_april2020
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_february2020
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_december2019
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_october2019
https://www.nxtbook.com/nxtbooks/sage/fai_201909
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_july2019
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_june2019
https://www.nxtbook.com/nxtbooks/sage/canadianpharmacistsjournal_05062019
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_april2019
https://www.nxtbook.com/nxtbooks/sage/sri_supplement_201903
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_february2019
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_december2018
https://www.nxtbook.com/nxtbooks/sage/tec_20180810
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_october2018
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_julyaugust2018
https://www.nxtbook.com/nxtbooks/sage/fai_201807
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_june2018
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_april2018
https://www.nxtbook.com/nxtbooks/sage/sri_supplement_201803
https://www.nxtbook.com/nxtbooks/sage/slas_discovery_201712
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_february2018
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_december2017
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_november2017
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_october2017
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_september2017
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_julyaugust2017
https://www.nxtbook.com/nxtbooks/sage/fai_supplement_201709
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_june2017
https://www.nxtbook.com/nxtbooks/sage/hospitalpharmacy_may2017
https://www.nxtbook.com/nxtbooks/sage/fai_201706
https://www.nxtbook.com/nxtbooks/sage/fai_201607
https://www.nxtbookmedia.com