Shaklee Product Guide 2015, Issue 2 - 132

CLINICAL RESEARCH // PROOF OF THE PRODUCTS 49. Chuck, C.S., et al., Postprandial glycemia on two sweet confections with different carbohydrate patterns. Fed Proc, 1982. 41(398). 50. Pattison, T., R. Allen, and H. Ashley, Automated testing of slowly changing levels of analytes in mixtures. Clin Chem, 1982. 28(7): p. 1613. 51. Soparkar, P.M., et al., Clinical study of the effect of an organic dentifrice on gingivitis and plaque formation. J Dent Res, 1980. 59(275). 52. Poovaiah, B.P., J.A. Rider, and J. Scala, The kinetics of vitamin C in human blood plasma from vitamin C supplements. Fed Proc, 1980. 39(3): p. 557. 53. Albanese, A.A., et al., Effects of calcium and micronutrients on premenopausal and postmenopausal bone loss. JAMA, 1980. 244(17): p. 1915. 54. Newman, M.B., et al., Clinical study of the effectiveness of an organic dentifrice for stain removal. American Associations for Dental Research, 1980. 59: p. 276. Proof of the Products* Nutrition Subject: 12-Hour Calcium Bioavailability Study This study assessed the serum and urine response during the initial 12 hours after the ingestion of a new calcium formulation found in Shaklee OsteoMatrix®. Changes in serum calcium were measured at nine time points (0 hr, 1 hr, 2 hr, 3 hr, 4 hr, 5 hr, 6 hr, 8 hr, and 12 hr) during the initial 12 hours following ingestion of two tablets containing a total of 500 mg calcium. At carefully timed intervals, within minutes of providing a baseline blood sample, 10 healthy volunteers ingested two supplement tablets with 125 mL of water. Results from the serum calcium assays on samples collected during the 12 hours immediately following supplementation were compared to each other and to the baseline serum calcium levels. The net changes in serum calcium during the 12 hours immediately following supplementation are presented graphically in the figure below: Serum Calcium (mg/dL) Serum Calcium (mg/dL) 0.6 0.4 0.2 0.0 Net Increase in Serum Calcium PLACEBO (FLAVORED WATER) 200 + PERFORMANCE 200 + 2 MINUTES TIL FATIGUE MINUTES CYCLING of max) 12 0 (45% to 75% VO2 max) 6 (80% 8 VO210 2 4 Time in Hours Time in Hours These results indicate that the net increase in serum calcium levels of the volunteers increased during the initial 5 hours after supplementation and then slowly decreased through the 12-hour monitoring period. A comparison of the net changes in serum calcium during the first 12 hours following supplementation (ANOVA) determined that significant differences in the net increase of serum calcium from baseline occurred from 2 to 12 postcalcium ingestion (P<.05). These data demonstrate that the calcium provided in OsteoMatrix is well absorbed during the initial 12 hours following ingestion. 132 This pilot study was designed to determine changes in weight loss, anthropometric measurements, and estimates of body composition in an uncontrolled 12-week trial with 27 moderately overweight, but otherwise healthy, men and women. Subjects were instructed to consume two Shaklee 180 beverage shakes, three Shaklee 180 dietary supplement tablets, one Shaklee 180 nutrition bar, and a healthy meal and/or snack daily. Eighteen participants completed the 12-week trial, of which 14 were fully compliant. Data from 14 participants show that subjects lost an average of 15.4 pounds and maintained their fat-free mass based on estimates from skinfold caliper measures. Together, these data suggest that weight loss was derived primarily from body fat and that fat-free mass was preserved. Waist and hip measurements were reduced by 4.1 and 2.6 inches, respectively, which was a significant reduction in waist-to-hip ratio, a predictor of cardiovascular disease. In addition, favorable changes were observed in blood lipids, including a 10% drop in total cholesterol and 34% drop in triglycerides. Additionally, no significant adverse events were noted by participants over the 12 weeks, and there were no significant changes in markers of renal and liver function or blood chemistries. These findings appear to support recent scientific evidence, which suggests that consumption of additional leucine during a weight-loss diet spares lean tissue. This trial also provides preliminary evidence that adherence to a novel weight-loss program for 12 weeks resulted in effective weight loss and improvements in anthropometric measurements. 110 105 Estimated Fat-Free Mass vs. Time PLACEBO (FLAVORED WATER) 200 + 2 100.5 100 95 90 190 185 99.4 PERFORMANCE 200 + MINUTES CYCLING 33 MINUTES TIL FATIGUE (45% Week 2 Week 4 Week 6(80% of VO2 max) 12 Baseline to 75% VO2 max) Week 8 Week 10 Week Mean Weight Loss vs. Time 186.8 PLACEBO (FLAVORED WATER) 200 PERFORMANCE 200 + 2 180 175 170 33 athletes at the rate of one liter per hour. Subject: Shaklee 180® Weight-Loss Program Clinical Study: Shaklee 180 12-Week Safety and Efficacy Pilot Study Fat-Free Mass, Pounds 48. Spallholz, J.E., et al., Cytotoxic activity of selenium compounds and glutathione peroxidase assessed by scanning electron microscopy. Fed Proc, 1982. 41(3): p. 529. Weight Management Weight, Pounds 47. Story, J.A., et al., Modification of human fecal steroid excretion by a dietary fiber supplement. Fed Proc, 1983. 42(4): p. 1062. MINUTES CYCLING + 33 171.4 MINUTES TIL FATIGUE (45% Week 2 Week 4 Week 6(80% of VO2 max) 12 Baseline to 75% VO2 max) Week 8 Week 10 Week Sports Nutrition Shaklee Performance maintained bloodglucose levels significantly higher than water throughout the test period, while maintaining plasma volume equal to that of water. Shaklee Performance also maintained a higher plasma osmolality than water, helping balance thirst with fluid needs and supporting the osmotic drive to drink. It was also the best-tolerated of all the carbohydrate-electrolyte prototypes. Clinical Study: Shaklee Performance- Maximizing Endurance Shaklee Performance helped maintain athletes' blood-glucose levels for muscle energy and fluid levels for proper hydration. After more than three hours of vigorous cycling in the laboratory, during which time the cyclists consumed either Shaklee Performance or water, the speed was turned up even higher. At this sprint pace, Shaklee Performance drinkers endured for 33 minutes, while the water-only group lasted just two minutes. Shaklee Performance Offers Proven Endurance PLACEBO (FLAVORED WATER) 200 + PERFORMANCE 200 + MINUTES CYCLING (45% to 75% VO2 max) 2 33 MINUTES UNTIL FATIGUE (80% of VO2 max) 1. Wong LG, Jensen CD, Whittam JH. The effects of sodium carbohydrate (Na:Carb) ratios in rehydration beverages (RB) on plasma glucose (PG), osmolality (PO), volume (PV), and subject tolerance (ST). FASEB J 1990;4:A381 (abstr). 2. Yaspelkis III BB, Patterson JG, Anderla PA, Ding Z, Ivy JL. Carbohydrate supplementation spares muscle glycogen during variable intensity exercise. J Appl Physiol 1993;75:1477-85. Subject: Shaklee Physique® Workout Maximizer Supplement Clinical Study: Physique-Maximizing Muscle Recovery for Serious Athletes Physique was tested with nine highly trained cyclists. During each of three test periods, athletes rode for two hours with an alternating workload that reflected racelike conditions. Immediately and two hours after every ride, athletes drank one of three drinks: Physique, with its unique carbohydrate-protein combination formula (40.5 g protein, 112.5 g carbohydrate); a protein drink reminiscent of the "muscle" drinks popular with body builders (40.5 g protein); and a carbohydrate drink similar to the "carboload" products on the market (112.5 g carbohydrate). These were consumed as 16 oz. beverages. Results of the study confirmed that the carbohydrate-protein combination of Physique elicited a greater insulin response in the blood than the carbohydrate or protein supplements alone. The greater insulin levels produced by Physique's carbohydrate-protein formula also led to a greater increase in the rate of muscle energy stored (glycogen) in the fourhour period following exercise than either the carbohydrate or protein supplements alone.1 Subject: Shaklee Performance® Maximum Endurance Sports Drink Clinical Study: Shaklee Performance- Effective Hydration and More Energy Cyclists rode stationary bicycles at 70% VO2 max for two to three hours. Shaklee Performance, other carbohydrate-electrolyte prototypes, and water were given to the *This section highlights important information that you may wish to review with your physician or health care professional.

Table of Contents for the Digital Edition of Shaklee Product Guide 2015, Issue 2

Contents
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Shaklee Product Guide 2015, Issue 2 - Cover3
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