Food Protection Trends - July 2008 - 468

ARTICLES Copyright© 2008, International Association for Food Protection 6200 Aurora Ave., Suite 200W, Des Moines, IA 50322-2864 Food Protection Trends, Vol. 28, No. 7, Pages 468–472 An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples JOHN WILLFORD1 and LAWRENCE D. GOODRIDGE2 * 1 Dept. of Animal Science, University of Wyoming, Laramie, WY 82071, USA; 2Dept. of Animal Sciences, Colorado State University, 350 West Pitkin St., Fort Collins, CO 80523-1171, USA SUMMARy INTRODUCTION Escherichia coli O157:H7 continues to be an important agent of foodborne illness, causing an estimated 70,000 illnesses, 2,000 hospitalizations, and 60 deaths annually in the United States (9). Fecally contaminated food and water are the sources of E. coli O157:H7 infections in humans (4). Cattle and other ruminants have been established as natural reservoirs for E. coli O157:H7, and these food production animals continue to play a role in the epidemiology of human infections (7). During 2007, there was a spike in E. coli O157:H7-related beef recalls. For example, approximately 30.1 million pounds of ground beef were recalled, and the implicated ground beef collectively resulted in at least 55 cases of E. coli O157:H7 illness (3). It is clear that there remains an acute need for the development of easy-to-use, robust, and sensitive detection methods for this pathogen. Also, although many methods have been developed to detect E. coli O157:H7 in food and water, most of the tests are not designed to be conducted in a field setting. The plethora of diagnostic methods that have been developed for detection of this organism The Phast Swab is a vertically integrated assay for rapid detection of Escherichia coli O157:H7. A sampling device, an E. coli O157:H7 reporter bacteriophage carrying the lacZ gene, bacterial growth medium, E. coli O157:H7-specific immunomagnetic beads and a β-galactosidase substrate (colorimetric or luminescent) are contained within a Snap Valve™ device. To conduct the assay, a sample to be tested is swabbed, and the swab is returned to the Snap Valve™ device; an eight-hour enrichment follows. Any E. coli O157:H7 cells are isolated and concentrated by immunomagnetic separation, and the reporter bacteriophage is added to the test compartment, where it infects any viable E. coli O157:H7 present in the sample, forcing the bacteria to make large amounts of β-galactosidase. Following the infection process (approximately 1 h), the cap of the Phast Swab is broken, releasing the substrate, which reacts with the β-galactosidase to produce a colorimetric signal (read visually) or a luminescent signal (read with a hand-held luminometer). The Phast Swab was evaluated for its ability to detect E. coli O157:H7 on 100 cm2 portions of beef. The assay was capable of detecting 103 CFU/100 cm2 of E. coli O157:H7 within 12 hours when a colorimetric substrate was used, and 101 CFU/100 cm2 within 10 hours with the use of a luminescent substrate. The nature of this detection method is such that it effectively detects E. coli O157:H7 in a simple and rapid manner, with minimal need for instrumentation to interpret the test result. A peer-reviewed article *Author for correspondence: 970.491.6271; Fax: 970.491.5326 Email: Lawrence.Goodridge@Colostate.edu 468 FOOD PROTECTION TRENDS | JULY 2008

Food Protection Trends - July 2008

Table of Contents for the Digital Edition of Food Protection Trends - July 2008

Food Protection Trends - July 2008
Contents
Sustaining Members
Lone Star Perspective from Your President
Commentary from the Executive Director
An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples
Listeria Prevention Practices for Small Cheese Operations
New Members
News
Industry Products
Award Winners
Committee Meetings
Ivan Parkin Lecture
John H. Silliker Lecture
Preliminary Program
Networking Opportunities
General Information
Registration Form
Workshops
Exhibitors
Special Contributors and Sponsors
Coming Events
Advertising Index
Journal of Food Protection Table of Contents
Audiovisual Library Order Form
Booklet Order Form
Membership Application
Food Protection Trends - July 2008 - Food Protection Trends - July 2008
Food Protection Trends - July 2008 - Cover2
Food Protection Trends - July 2008 - 453
Food Protection Trends - July 2008 - Contents
Food Protection Trends - July 2008 - 455
Food Protection Trends - July 2008 - 456
Food Protection Trends - July 2008 - 457
Food Protection Trends - July 2008 - 458
Food Protection Trends - July 2008 - 459
Food Protection Trends - July 2008 - 460
Food Protection Trends - July 2008 - Sustaining Members
Food Protection Trends - July 2008 - 462
Food Protection Trends - July 2008 - 463
Food Protection Trends - July 2008 - Lone Star Perspective from Your President
Food Protection Trends - July 2008 - 465
Food Protection Trends - July 2008 - Commentary from the Executive Director
Food Protection Trends - July 2008 - 467
Food Protection Trends - July 2008 - An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples
Food Protection Trends - July 2008 - 469
Food Protection Trends - July 2008 - 470
Food Protection Trends - July 2008 - 471
Food Protection Trends - July 2008 - 472
Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations
Food Protection Trends - July 2008 - 474
Food Protection Trends - July 2008 - 475
Food Protection Trends - July 2008 - 476
Food Protection Trends - July 2008 - 477
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Food Protection Trends - July 2008 - 479
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Food Protection Trends - July 2008 - 481
Food Protection Trends - July 2008 - 482
Food Protection Trends - July 2008 - 483
Food Protection Trends - July 2008 - New Members
Food Protection Trends - July 2008 - 485
Food Protection Trends - July 2008 - 486
Food Protection Trends - July 2008 - News
Food Protection Trends - July 2008 - 488
Food Protection Trends - July 2008 - 489
Food Protection Trends - July 2008 - 490
Food Protection Trends - July 2008 - Industry Products
Food Protection Trends - July 2008 - 492
Food Protection Trends - July 2008 - 493
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Food Protection Trends - July 2008 - 496
Food Protection Trends - July 2008 - 497
Food Protection Trends - July 2008 - 498
Food Protection Trends - July 2008 - 499
Food Protection Trends - July 2008 - Award Winners
Food Protection Trends - July 2008 - Committee Meetings
Food Protection Trends - July 2008 - Ivan Parkin Lecture
Food Protection Trends - July 2008 - John H. Silliker Lecture
Food Protection Trends - July 2008 - Preliminary Program
Food Protection Trends - July 2008 - 505
Food Protection Trends - July 2008 - 506
Food Protection Trends - July 2008 - 507
Food Protection Trends - July 2008 - 508
Food Protection Trends - July 2008 - 509
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Food Protection Trends - July 2008 - 530
Food Protection Trends - July 2008 - 531
Food Protection Trends - July 2008 - 532
Food Protection Trends - July 2008 - 533
Food Protection Trends - July 2008 - 534
Food Protection Trends - July 2008 - Networking Opportunities
Food Protection Trends - July 2008 - General Information
Food Protection Trends - July 2008 - Registration Form
Food Protection Trends - July 2008 - Workshops
Food Protection Trends - July 2008 - 539
Food Protection Trends - July 2008 - Exhibitors
Food Protection Trends - July 2008 - 541
Food Protection Trends - July 2008 - 542
Food Protection Trends - July 2008 - 543
Food Protection Trends - July 2008 - Special Contributors and Sponsors
Food Protection Trends - July 2008 - 545
Food Protection Trends - July 2008 - Coming Events
Food Protection Trends - July 2008 - Advertising Index
Food Protection Trends - July 2008 - Journal of Food Protection Table of Contents
Food Protection Trends - July 2008 - 549
Food Protection Trends - July 2008 - Audiovisual Library Order Form
Food Protection Trends - July 2008 - Booklet Order Form
Food Protection Trends - July 2008 - Membership Application
Food Protection Trends - July 2008 - Cover3
Food Protection Trends - July 2008 - Cover4
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