Food Protection Trends - July 2008 - (Page 470) FIGURE 2. Completion of the Phast Swab assay. The swab is removed, the surface to be tested is swabbed, and the swab is returned to the device; eight-hour enrichment follows (Fig. 2A). After enrichment, the IMS beads (with E. coli O157:H7 cells attached) are concentrated, and the growth media is removed (Fig. 2B). Following a wash step, the reporter phage is added (107 PFU/ml) and the Phast Swab is incubated at 37oC for 1.5 hours (Fig. 2C). Finally, the cap of the Phast Swab is broken, releasing the β-galactosidase (CPRG) substrate into the bottom of the device (Fig. 2D), where it will react with any β-galactosidase present. A positive test is indicated by the development of a red color; in a negative test, the color remains yellow (Fig. 2E). FIGURE 3. Results of the colorimetric swab assay. The detection limit was an initial inoculum of 103 CFU/100 cm2. Key: C1, cell-only negative control; C2, phage-only negative control. The initial inoculum (in CFU/100 cm2) is indicated beneath each Phast Swab. A positive test result is indicated by the development of a red (or orange) color; in a negative test, the color remains yellow. transfer pipette (Fig. 2B). Following a wash step (in PBS-Tween), the reporter phage was added at a concentration of 107 PFU/ml, and the Phast Swab was incubated at 37oC for 1.5 hours (Fig. 2C). Finally, the cap of the Phast Swab was broken, releasing the β-galactosidase substrate into the bottom of the device (Fig. 2D), followed by a 2-hour incubation at 37 oC for the colorimetric substrate or a 30-minute incubation at 37oC for the luminescent substrate. For colorimetric detection, a positive test was determined visually, and indicated by the development of a red color, while in a negative test, the color remained yellow (Fig. 2E). For luminescent detection, the incubation 470 FOOD PROTECTION TRENDS | JULY 2008
Table of Contents Feed for the Digital Edition of Food Protection Trends - July 2008 Food Protection Trends - July 2008 Contents Sustaining Members Lone Star Perspective from Your President Commentary from the Executive Director An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples Listeria Prevention Practices for Small Cheese Operations New Members News Industry Products Award Winners Committee Meetings Ivan Parkin Lecture John H. Silliker Lecture Preliminary Program Networking Opportunities General Information Registration Form Workshops Exhibitors Special Contributors and Sponsors Coming Events Advertising Index Journal of Food Protection Table of Contents Audiovisual Library Order Form Booklet Order Form Membership Application Food Protection Trends - July 2008 Food Protection Trends - July 2008 - Food Protection Trends - July 2008 (Page Cover1) Food Protection Trends - July 2008 - Food Protection Trends - July 2008 (Page Cover2) Food Protection Trends - July 2008 - Food Protection Trends - July 2008 (Page 453) Food Protection Trends - July 2008 - Contents (Page 454) Food Protection Trends - July 2008 - Contents (Page 455) Food Protection Trends - July 2008 - Contents (Page 456) Food Protection Trends - July 2008 - Contents (Page 457) Food Protection Trends - July 2008 - Contents (Page 458) Food Protection Trends - July 2008 - Contents (Page 459) Food Protection Trends - July 2008 - Contents (Page 460) Food Protection Trends - July 2008 - Sustaining Members (Page 461) Food Protection Trends - July 2008 - Sustaining Members (Page 462) Food Protection Trends - July 2008 - Sustaining Members (Page 463) Food Protection Trends - July 2008 - Lone Star Perspective from Your President (Page 464) Food Protection Trends - July 2008 - Lone Star Perspective from Your President (Page 465) Food Protection Trends - July 2008 - Commentary from the Executive Director (Page 466) Food Protection Trends - July 2008 - Commentary from the Executive Director (Page 467) Food Protection Trends - July 2008 - An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples (Page 468) Food Protection Trends - July 2008 - An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples (Page 469) Food Protection Trends - July 2008 - An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples (Page 470) Food Protection Trends - July 2008 - An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples (Page 471) Food Protection Trends - July 2008 - An Integrated Assay for Rapid Detection of Escherichia coli O157:H7 on Beef Samples (Page 472) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 473) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 474) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 475) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 476) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 477) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 478) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 479) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 480) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 481) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 482) Food Protection Trends - July 2008 - Listeria Prevention Practices for Small Cheese Operations (Page 483) Food Protection Trends - July 2008 - New Members (Page 484) Food Protection Trends - July 2008 - New Members (Page 485) Food Protection Trends - July 2008 - New Members (Page 486) Food Protection Trends - July 2008 - News (Page 487) Food Protection Trends - July 2008 - News (Page 488) Food Protection Trends - July 2008 - News (Page 489) Food Protection Trends - July 2008 - News (Page 490) Food Protection Trends - July 2008 - Industry Products (Page 491) Food Protection Trends - July 2008 - Industry Products (Page 492) Food Protection Trends - July 2008 - Industry Products (Page 493) Food Protection Trends - July 2008 - Industry Products (Page 494) Food Protection Trends - July 2008 - Industry Products (Page 495) Food Protection Trends - July 2008 - Industry Products (Page 496) Food Protection Trends - July 2008 - Industry Products (Page 497) Food Protection Trends - July 2008 - Industry Products (Page 498) Food Protection Trends - July 2008 - Industry Products (Page 499) Food Protection Trends - July 2008 - Award Winners (Page 500) Food Protection Trends - July 2008 - Committee Meetings (Page 501) Food Protection Trends - July 2008 - Ivan Parkin Lecture (Page 502) Food Protection Trends - July 2008 - John H. Silliker Lecture (Page 503) Food Protection Trends - July 2008 - Preliminary Program (Page 504) Food Protection Trends - July 2008 - Preliminary Program (Page 505) Food Protection Trends - July 2008 - Preliminary Program (Page 506) Food Protection Trends - July 2008 - Preliminary Program (Page 507) Food Protection Trends - July 2008 - Preliminary Program (Page 508) Food Protection Trends - July 2008 - Preliminary Program (Page 509) Food Protection Trends - July 2008 - Preliminary Program (Page 510) Food Protection Trends - July 2008 - Preliminary Program (Page 511) Food Protection Trends - July 2008 - Preliminary Program (Page 512) Food Protection Trends - July 2008 - Preliminary Program (Page 513) Food Protection Trends - July 2008 - Preliminary Program (Page 514) Food Protection Trends - July 2008 - Preliminary Program (Page 515) Food Protection Trends - July 2008 - Preliminary Program (Page 516) Food Protection Trends - July 2008 - Preliminary Program (Page 517) Food Protection Trends - July 2008 - Preliminary Program (Page 518) Food Protection Trends - July 2008 - Preliminary Program (Page 519) Food Protection Trends - July 2008 - Preliminary Program (Page 520) Food Protection Trends - July 2008 - Preliminary Program (Page 521) Food Protection Trends - July 2008 - Preliminary Program (Page 522) Food Protection Trends - July 2008 - Preliminary Program (Page 523) Food Protection Trends - July 2008 - Preliminary Program (Page 524) Food Protection Trends - July 2008 - Preliminary Program (Page 525) Food Protection Trends - July 2008 - Preliminary Program (Page 526) Food Protection Trends - July 2008 - Preliminary Program (Page 527) Food Protection Trends - July 2008 - Preliminary Program (Page 528) Food Protection Trends - July 2008 - Preliminary Program (Page 529) Food Protection Trends - July 2008 - Preliminary Program (Page 530) Food Protection Trends - July 2008 - Preliminary Program (Page 531) Food Protection Trends - July 2008 - Preliminary Program (Page 532) Food Protection Trends - July 2008 - Preliminary Program (Page 533) Food Protection Trends - July 2008 - Preliminary Program (Page 534) Food Protection Trends - July 2008 - Networking Opportunities (Page 535) Food Protection Trends - July 2008 - General Information (Page 536) Food Protection Trends - July 2008 - Registration Form (Page 537) Food Protection Trends - July 2008 - Workshops (Page 538) Food Protection Trends - July 2008 - Workshops (Page 539) Food Protection Trends - July 2008 - Exhibitors (Page 540) Food Protection Trends - July 2008 - Exhibitors (Page 541) Food Protection Trends - July 2008 - Exhibitors (Page 542) Food Protection Trends - July 2008 - Exhibitors (Page 543) Food Protection Trends - July 2008 - Special Contributors and Sponsors (Page 544) Food Protection Trends - July 2008 - Special Contributors and Sponsors (Page 545) Food Protection Trends - July 2008 - Coming Events (Page 546) Food Protection Trends - July 2008 - Advertising Index (Page 547) Food Protection Trends - July 2008 - Journal of Food Protection Table of Contents (Page 548) Food Protection Trends - July 2008 - Journal of Food Protection Table of Contents (Page 549) Food Protection Trends - July 2008 - Audiovisual Library Order Form (Page 550) Food Protection Trends - July 2008 - Booklet Order Form (Page 551) Food Protection Trends - July 2008 - Membership Application (Page 552) Food Protection Trends - July 2008 - Membership Application (Page Cover3) Food Protection Trends - July 2008 - Membership Application (Page Cover4)
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