Food Protection Trends - November/December 2017 - 421

Figure 1. The carcass model. A: Flanks from sheep (M. obliquus externus abdominis) were
placed over the pipe frame. B: The flanks were placed with the outer surface of the meat facing
outwards, and attached tightly in place over the pipe frame with the aid of two or three cable ties.

Preparation of inocula
The inoculum used at different dilutions was comprised
of three strains of Enterobacteriaceae associated with sheep
carcasses (34), namely Citrobacter freundii (CCUG 418),
Enterobacter cloacae (CCUG 6323), and Escherichia coli
(CCUG 17620). The strains were kept in glycerol stocks
at below -75°C. Prior to preparation of the inoculum, the
strains were plated from the stocks on blood agar (BA) plates
and incubated overnight at 37°C ± 1°C. One well-isolated
colony from each strain was inoculated separately into 9 mL
of buffered peptone water and incubated at 37° ± 1°C for
24 h. For estimation of the number of bacteria, the cultures
were serially diluted in peptone salt diluent (0.1% peptone,
0.9% NaCl) and 100 µl from the appropriate dilutions
were plated onto BA in triplicate. In order to achieve the
required concentration, an equal volume of the appropriate
concentrations for each strain were mixed and kept on ice
until use. For the pilot study, two inocula were prepared;
(high) 2 × 106 colony-forming units (CFU)/mL, and (low) 2
× 103 CFU/mL. For the main and confirmatory experiments,
inocula of estimates of moderate concentrations were used,
2.6 × 104 CFU/mL and 2.8 × 104 CFU/mL, respectively.
In all three experimental rounds, 500 µL was inoculated
onto the surface of each meat tissue model and spread
out evenly on an area of 100 cm2, using a sterile L-shaped
spreader. Inoculated samples were left on the bench to dry
for a few minutes, before they were either a) sampled 2 h
after inoculation, without chilling or b) stored for 12 h at
4 ± 0.5°C (only the pilot) or c) stored for 24 h at 4 ± 0.5°C.
Sampling of the carcass model
The sampling techniques investigated in this study were
chosen on the basis of the techniques used by fifteen different

abattoirs from EU and EEA countries participating in the Hygenea project. A specially made, circular cutting support board
(approximately 130 mm in diameter and 4 cm thick) that fitted
within the model pipe frame was used to ensure that the meat
model did not dip into the hollow pipe during sampling.
After sampling, the excised meat samples and the swabs
were placed in separate stomacher bags or tubes and kept refrigerated (4 ± 0.5°C) until being transported to the laboratory at the Norwegian Veterinary Institute for microbiological
analysis the following day.
Destructive method; excision (A). A circular incision
(25 mm in diameter) was made in the surface of the meat
tissue model with a sterile coring tool with a circular blade
(BeefSteaker, Bürkle, Germany) (Fig. 2A). The disc of tissue
(approximately 5 cm2 and 2 mm thick) was removed from
the tool blade with a sterile scalpel and forceps and placed
in a labelled sterile stomacher bag.
Non-destructive methods. For all four of the non-destructive methods used, the test area on the carcass model was
delineated by using a single-use, square template frame
made of paper (100 cm2) that was laid over the model, and
the area within the template frame was sampled.
Gauze cloth swab (B). One sterile medical gauze cloth swab
(10 × 10 cm) (Mesosoft, Mölnlycke Health Care AB, Sweden)
was added to 10 mL sterile peptone salt diluent. The test area
defined by the template was swabbed, using 10 horizontal and
10 vertical movements (approx. 20 s) (Fig. 2B).
Sponge swab (C). The test area defined by the template was
swabbed with a pre-moistened Polywipe (5 × 10 cm) (Medical
Wire & Equipment, Wiltshire, UK), using 10 horizontal and
10 vertical movements (approx. 20 s) (Fig. 2C).
Sponge-Stick (D). The test area defined by the template
was swabbed with a pre-moistened Sponge-Stick (3.75 × 7.5

November/December Food Protection Trends

421



Table of Contents for the Digital Edition of Food Protection Trends - November/December 2017

The Cleanliness of Resusable Water Bottles: How Contamination Levels are Affected by Bottle Usage and Cleaning Behaviors of Bottle Owners
Impact of Carcass Anatomical Location on the Microbiological Profile of Beef Trimmings
Thermal Processing Parameters to Ensure a 5-log Reduction of Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in Acidified Tomato-based Foods
Experimental Evaluation of Performance of Sampling Techniques for Microbiological Quantification on Carcass Services
Effect-based Analytics for Toxicological Screening - Concepts for Future Developments
Beyond the Bio - John Luchansky
PDF Highlight - Food Chemical Hazards and Food Allergy PDG
IAFP 2017 In Review
Industry Products
Coming Events
Food Protection Trends - November/December 2017 - Cover1
Food Protection Trends - November/December 2017 - Cover2
Food Protection Trends - November/December 2017 - 385
Food Protection Trends - November/December 2017 - 386
Food Protection Trends - November/December 2017 - 387
Food Protection Trends - November/December 2017 - 388
Food Protection Trends - November/December 2017 - 389
Food Protection Trends - November/December 2017 - 390
Food Protection Trends - November/December 2017 - 391
Food Protection Trends - November/December 2017 - The Cleanliness of Resusable Water Bottles: How Contamination Levels are Affected by Bottle Usage and Cleaning Behaviors of Bottle Owners
Food Protection Trends - November/December 2017 - 393
Food Protection Trends - November/December 2017 - 394
Food Protection Trends - November/December 2017 - 395
Food Protection Trends - November/December 2017 - 396
Food Protection Trends - November/December 2017 - 397
Food Protection Trends - November/December 2017 - 398
Food Protection Trends - November/December 2017 - 399
Food Protection Trends - November/December 2017 - 400
Food Protection Trends - November/December 2017 - 401
Food Protection Trends - November/December 2017 - 402
Food Protection Trends - November/December 2017 - Impact of Carcass Anatomical Location on the Microbiological Profile of Beef Trimmings
Food Protection Trends - November/December 2017 - 404
Food Protection Trends - November/December 2017 - 405
Food Protection Trends - November/December 2017 - 406
Food Protection Trends - November/December 2017 - 407
Food Protection Trends - November/December 2017 - 408
Food Protection Trends - November/December 2017 - Thermal Processing Parameters to Ensure a 5-log Reduction of Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in Acidified Tomato-based Foods
Food Protection Trends - November/December 2017 - 410
Food Protection Trends - November/December 2017 - 411
Food Protection Trends - November/December 2017 - 412
Food Protection Trends - November/December 2017 - 413
Food Protection Trends - November/December 2017 - 414
Food Protection Trends - November/December 2017 - 415
Food Protection Trends - November/December 2017 - 416
Food Protection Trends - November/December 2017 - 417
Food Protection Trends - November/December 2017 - 418
Food Protection Trends - November/December 2017 - Experimental Evaluation of Performance of Sampling Techniques for Microbiological Quantification on Carcass Services
Food Protection Trends - November/December 2017 - 420
Food Protection Trends - November/December 2017 - 421
Food Protection Trends - November/December 2017 - 422
Food Protection Trends - November/December 2017 - 423
Food Protection Trends - November/December 2017 - 424
Food Protection Trends - November/December 2017 - 425
Food Protection Trends - November/December 2017 - 426
Food Protection Trends - November/December 2017 - 427
Food Protection Trends - November/December 2017 - 428
Food Protection Trends - November/December 2017 - 429
Food Protection Trends - November/December 2017 - Effect-based Analytics for Toxicological Screening - Concepts for Future Developments
Food Protection Trends - November/December 2017 - 431
Food Protection Trends - November/December 2017 - 432
Food Protection Trends - November/December 2017 - 433
Food Protection Trends - November/December 2017 - 434
Food Protection Trends - November/December 2017 - 435
Food Protection Trends - November/December 2017 - 436
Food Protection Trends - November/December 2017 - 437
Food Protection Trends - November/December 2017 - Beyond the Bio - John Luchansky
Food Protection Trends - November/December 2017 - 439
Food Protection Trends - November/December 2017 - 440
Food Protection Trends - November/December 2017 - PDF Highlight - Food Chemical Hazards and Food Allergy PDG
Food Protection Trends - November/December 2017 - 442
Food Protection Trends - November/December 2017 - 443
Food Protection Trends - November/December 2017 - 444
Food Protection Trends - November/December 2017 - IAFP 2017 In Review
Food Protection Trends - November/December 2017 - 446
Food Protection Trends - November/December 2017 - 447
Food Protection Trends - November/December 2017 - 448
Food Protection Trends - November/December 2017 - 449
Food Protection Trends - November/December 2017 - 450
Food Protection Trends - November/December 2017 - 451
Food Protection Trends - November/December 2017 - 452
Food Protection Trends - November/December 2017 - 453
Food Protection Trends - November/December 2017 - 454
Food Protection Trends - November/December 2017 - 455
Food Protection Trends - November/December 2017 - 456
Food Protection Trends - November/December 2017 - 457
Food Protection Trends - November/December 2017 - 458
Food Protection Trends - November/December 2017 - 459
Food Protection Trends - November/December 2017 - 460
Food Protection Trends - November/December 2017 - 461
Food Protection Trends - November/December 2017 - 462
Food Protection Trends - November/December 2017 - 463
Food Protection Trends - November/December 2017 - 464
Food Protection Trends - November/December 2017 - 465
Food Protection Trends - November/December 2017 - 466
Food Protection Trends - November/December 2017 - 467
Food Protection Trends - November/December 2017 - 468
Food Protection Trends - November/December 2017 - 469
Food Protection Trends - November/December 2017 - 470
Food Protection Trends - November/December 2017 - 471
Food Protection Trends - November/December 2017 - 472
Food Protection Trends - November/December 2017 - 473
Food Protection Trends - November/December 2017 - 474
Food Protection Trends - November/December 2017 - 475
Food Protection Trends - November/December 2017 - 476
Food Protection Trends - November/December 2017 - 477
Food Protection Trends - November/December 2017 - 478
Food Protection Trends - November/December 2017 - 479
Food Protection Trends - November/December 2017 - 480
Food Protection Trends - November/December 2017 - 481
Food Protection Trends - November/December 2017 - 482
Food Protection Trends - November/December 2017 - 483
Food Protection Trends - November/December 2017 - 484
Food Protection Trends - November/December 2017 - 485
Food Protection Trends - November/December 2017 - 486
Food Protection Trends - November/December 2017 - 487
Food Protection Trends - November/December 2017 - 488
Food Protection Trends - November/December 2017 - 489
Food Protection Trends - November/December 2017 - 490
Food Protection Trends - November/December 2017 - 491
Food Protection Trends - November/December 2017 - 492
Food Protection Trends - November/December 2017 - 493
Food Protection Trends - November/December 2017 - 494
Food Protection Trends - November/December 2017 - 495
Food Protection Trends - November/December 2017 - 496
Food Protection Trends - November/December 2017 - 497
Food Protection Trends - November/December 2017 - 498
Food Protection Trends - November/December 2017 - 499
Food Protection Trends - November/December 2017 - 500
Food Protection Trends - November/December 2017 - 501
Food Protection Trends - November/December 2017 - 502
Food Protection Trends - November/December 2017 - 503
Food Protection Trends - November/December 2017 - 504
Food Protection Trends - November/December 2017 - 505
Food Protection Trends - November/December 2017 - 506
Food Protection Trends - November/December 2017 - 507
Food Protection Trends - November/December 2017 - 508
Food Protection Trends - November/December 2017 - 509
Food Protection Trends - November/December 2017 - 510
Food Protection Trends - November/December 2017 - 511
Food Protection Trends - November/December 2017 - 512
Food Protection Trends - November/December 2017 - 513
Food Protection Trends - November/December 2017 - 514
Food Protection Trends - November/December 2017 - 515
Food Protection Trends - November/December 2017 - 516
Food Protection Trends - November/December 2017 - 517
Food Protection Trends - November/December 2017 - 518
Food Protection Trends - November/December 2017 - 519
Food Protection Trends - November/December 2017 - 520
Food Protection Trends - November/December 2017 - 521
Food Protection Trends - November/December 2017 - 522
Food Protection Trends - November/December 2017 - 523
Food Protection Trends - November/December 2017 - 524
Food Protection Trends - November/December 2017 - 525
Food Protection Trends - November/December 2017 - 526
Food Protection Trends - November/December 2017 - 527
Food Protection Trends - November/December 2017 - 528
Food Protection Trends - November/December 2017 - 529
Food Protection Trends - November/December 2017 - 530
Food Protection Trends - November/December 2017 - 531
Food Protection Trends - November/December 2017 - 532
Food Protection Trends - November/December 2017 - 533
Food Protection Trends - November/December 2017 - Industry Products
Food Protection Trends - November/December 2017 - 535
Food Protection Trends - November/December 2017 - 536
Food Protection Trends - November/December 2017 - 537
Food Protection Trends - November/December 2017 - 538
Food Protection Trends - November/December 2017 - 539
Food Protection Trends - November/December 2017 - 540
Food Protection Trends - November/December 2017 - 541
Food Protection Trends - November/December 2017 - 542
Food Protection Trends - November/December 2017 - 543
Food Protection Trends - November/December 2017 - Coming Events
Food Protection Trends - November/December 2017 - Cover3
Food Protection Trends - November/December 2017 - Cover4
http://www.nxtbook.com/nxtbooks/trilix/fpt_20190506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20190304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20190102
http://www.nxtbook.com/nxtbooks/trilix/fpt_20181112
http://www.nxtbook.com/nxtbooks/trilix/fpt_20180910
http://www.nxtbook.com/nxtbooks/trilix/fpt_20180708
http://www.nxtbook.com/nxtbooks/trilix/fpt_20180506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20180304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20180102
http://www.nxtbook.com/nxtbooks/trilix/fpt_20171112
http://www.nxtbook.com/nxtbooks/trilix/fpt_20170910
http://www.nxtbook.com/nxtbooks/trilix/fpt_20170708
http://www.nxtbook.com/nxtbooks/trilix/fpt_20170506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20170304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20170102
http://www.nxtbook.com/nxtbooks/trilix/fpt_20161112
http://www.nxtbook.com/nxtbooks/trilix/fpt_20160910
http://www.nxtbook.com/nxtbooks/trilix/fpt_20160708
http://www.nxtbook.com/nxtbooks/trilix/fpt_20160506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20160304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20160102
http://www.nxtbook.com/nxtbooks/trilix/fpt_20151112
http://www.nxtbook.com/nxtbooks/trilix/fpt_20150910
http://www.nxtbook.com/nxtbooks/trilix/fpt_20150708
http://www.nxtbook.com/nxtbooks/trilix/fpt_20150506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20150304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20150102
http://www.nxtbook.com/nxtbooks/trilix/fpt_20141112
http://www.nxtbook.com/nxtbooks/trilix/fpt_20140910
http://www.nxtbook.com/nxtbooks/trilix/fpt_20140708
http://www.nxtbook.com/nxtbooks/trilix/fpt_20140506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20140304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20140102
http://www.nxtbook.com/nxtbooks/trilix/fpt_20131112
http://www.nxtbook.com/nxtbooks/trilix/fpt_20130910
http://www.nxtbook.com/nxtbooks/trilix/fpt_20130708
http://www.nxtbook.com/nxtbooks/trilix/fpt_20130506
http://www.nxtbook.com/nxtbooks/trilix/fpt_20130304
http://www.nxtbook.com/nxtbooks/trilix/fpt_20130102
http://www.nxtbook.com/nxtbooks/trilix/fpt_201212
http://www.nxtbook.com/nxtbooks/trilix/fpt_201211
http://www.nxtbook.com/nxtbooks/trilix/fpt_201210
http://www.nxtbook.com/nxtbooks/trilix/fpt_201209
http://www.nxtbook.com/nxtbooks/trilix/fpt_201208
http://www.nxtbook.com/nxtbooks/trilix/fpt_201207
http://www.nxtbook.com/nxtbooks/trilix/fpt_201206
http://www.nxtbook.com/nxtbooks/trilix/fpt_201205
http://www.nxtbook.com/nxtbooks/trilix/fpt_201204
http://www.nxtbook.com/nxtbooks/trilix/fpt_201203
http://www.nxtbook.com/nxtbooks/trilix/fpt_201202
http://www.nxtbook.com/nxtbooks/trilix/fpt_201201
http://www.nxtbook.com/nxtbooks/trilix/fpt_201112
http://www.nxtbook.com/nxtbooks/trilix/fpt_201111
http://www.nxtbook.com/nxtbooks/trilix/fpt_201110
http://www.nxtbook.com/nxtbooks/trilix/fpt_201109
http://www.nxtbook.com/nxtbooks/trilix/fpt_201108
http://www.nxtbook.com/nxtbooks/trilix/fpt_201107
http://www.nxtbook.com/nxtbooks/trilix/fpt_201106
http://www.nxtbook.com/nxtbooks/trilix/fpt_201105
http://www.nxtbook.com/nxtbooks/trilix/fpt_201104
http://www.nxtbook.com/nxtbooks/trilix/fpt_201103
http://www.nxtbook.com/nxtbooks/trilix/fpt_201102
http://www.nxtbook.com/nxtbooks/trilix/fpt_201101
http://www.nxtbook.com/nxtbooks/trilix/fpt_201012
http://www.nxtbook.com/nxtbooks/trilix/fpt_201011
http://www.nxtbook.com/nxtbooks/trilix/fpt_201010
http://www.nxtbook.com/nxtbooks/trilix/fpt_201009
http://www.nxtbook.com/nxtbooks/trilix/fpt_201008
http://www.nxtbook.com/nxtbooks/trilix/fpt_201007
http://www.nxtbook.com/nxtbooks/trilix/fpt_201006
http://www.nxtbook.com/nxtbooks/trilix/fpt_201005
http://www.nxtbook.com/nxtbooks/trilix/fpt_201004
http://www.nxtbook.com/nxtbooks/trilix/fpt_201003
http://www.nxtbook.com/nxtbooks/trilix/fpt_201002
http://www.nxtbook.com/nxtbooks/trilix/fpt_201001
http://www.nxtbook.com/nxtbooks/trilix/fpt_200912
http://www.nxtbook.com/nxtbooks/trilix/fpt_200911
http://www.nxtbook.com/nxtbooks/trilix/fpt_200910
http://www.nxtbook.com/nxtbooks/trilix/fpt_200909
http://www.nxtbook.com/nxtbooks/trilix/fpt_200908
http://www.nxtbook.com/nxtbooks/trilix/fpt_200907
http://www.nxtbook.com/nxtbooks/trilix/fpt_200906
http://www.nxtbook.com/nxtbooks/trilix/fpt_200905
http://www.nxtbook.com/nxtbooks/trilix/fpt_200904
http://www.nxtbook.com/nxtbooks/trilix/fpt_200903
http://www.nxtbook.com/nxtbooks/trilix/fpt_200902
http://www.nxtbook.com/nxtbooks/trilix/fpt_200901
http://www.nxtbook.com/nxtbooks/trilix/fpt_200812
http://www.nxtbook.com/nxtbooks/trilix/fpt_200811
http://www.nxtbook.com/nxtbooks/trilix/fpt_200810
http://www.nxtbook.com/nxtbooks/trilix/fpt_200809
http://www.nxtbook.com/nxtbooks/trilix/fpt_200808
http://www.nxtbook.com/nxtbooks/trilix/fpt_200807
http://www.nxtbookMEDIA.com