Journal of Oral Implantology September 2012 - (Page 511)

RESEARCH Development of a Rat Model of Bisphosphonate-Related Osteonecrosis of the Jaw (BRONJ) Karen L. Marino, DDS, MS1,2 Ibrahim Zakhary, PhD1 Rafik A. Abdelsayed, DDS, MS3 Jared A. Carter4 Jack C. O’Neill4 Rania M Khashaba, DDS, PhD5 Mohammed Elsalanty, MD, PhD1 Mark R. Stevens, DDS6 James L. Borke, PhD7* The purpose of this study was to develop a rat model predictive of bisphosphonate-related osteonecrosis of the jaw (BRONJ) after exodontias. Thirty female rats were randomized into 2 groups, control and experimental. The experimental group received 2 intravenous injections of zoledronate (20 lg/kg). The mesial root of the right mandibular first molar was extracted. Rats were euthanized at 0, 4, and 8 weeks. Bone mineral density (BMD), collagen breakdown (pyridinium [PYD]), vascular regeneration (VEGF), and histology were examined. A trend toward higher PYD values was suggested in control vs experimental groups after wounding. Serum VEGF increased significantly after wounding for both control and experimental groups. After 8 weeks, VEGF continued to rise for the experimental group only. In the extraction socket area, BMD was significantly lower after wounding in control vs. zoledronate-treated rats. Histology sections from experimental groups showed bacteria and bone necrosis. Consistent findings of BRONJ features similar to those in humans were observed after zoledronate treatment Key Words: bisphosphonate, osteonecrosis of the jaw, rat model, BRONJ INTRODUCTION 1 Department of Oral Biology, Georgia Health Sciences University, College of Dental Medicine, Augusta, Ga. 2 Department of Periodontology, Georgia Health Sciences University, College of Dental Medicine, Augusta, Ga. 3 Department of Oral and Maxillofacial Pathology, Georgia Health Sciences University, College of Dental Medicine, Augusta, Ga. 4 Georgia Health Sciences University, College of Dental Medicine, Augusta, Ga. 5 Department of Orthopedic Surgery, Medical College of Georgia, Georgia Health Sciences University, Augusta, Ga. 6 Department of Oral and Maxillofacial Surgery, Georgia Health Sciences University, College of Dental Medicine, Augusta, Ga. 7 College of Dental Medicine, Western University of Health Sciences, Pomona, Calif; previously with Georgia Health Sciences University, College of Dental Medicine. * Corresponding author, e-mail: jborke@westernu.edu DOI: 10.1563/AAID-JOI-D-11-00057 isphosphonates are powerful compounds that have been successfully used for the treatment of complications of bone metastasis, including pathologic fractures, malignant hypercalcemia, osteoporosis, osteopenia, and Paget’s disease. Since 2003, case series reports began to emerge of osteonecrosis of the jaw associated with the use of bisphosphonates, primarily in patients with cancer.1,2 The most important predisposing factors for the development of bisphosphonate-related osteonecrosis of the jaw (BRONJ) appear to be the type and total dose of bisphosphonate, history of trauma, dental surgery, and dental infection.3 Time of exposure to bisphosphonates also appears to be Journal of Oral Implantology 511 B

Table of Contents for the Digital Edition of Journal of Oral Implantology September 2012

American Academy of Implant Dentistry Foundation Supports Basic and Clinical Research
Evaluation of 3- to 8-Year Treatment Outcomes and Success Rates With 6 Implant Brands in Partially Edentulous Patients
Histometric Analysis of Bone Repair in Bone-Implant Interface Using a Polylactic/ Polyglycolic Acid Copolymer Associated With Implants in Rabbit Tibia
Fold-Over Flap Technique for Developing the Facial Gingival Contour: A Case Report
Rehabilitation of the Atrophic Posterior Maxilla With Pterygoid Implants: A Review
Immediate Loading of Maxillary and Mandibular Implant-Supported Fixed Complete Dentures: A 1- to 10-Year Retrospective Study
Immediate Loading of Single Post-Extractive Implants in the Anterior Maxilla: 12-Month Results From a Multicenter Clinical Study
Immediate Implants After Enucleation of an Odontogenic Keratocyst: An Early Return to Function
Effects of Different Abutment Connection Designs on the Stress Distribution Around Five Different Implants: A 3-Dimensional Finite Element Analysis
Implant-Prosthetic Rehabilitation of a Patient with Nonsyndromic Oligodontia: A Clinical Report
Planning for Immediate Loading of Implant-Supported Prostheses: Literature Review
Development of a Rat Model of Bisphosphonate-Related Osteonecrosis of the Jaw (BRONJ)
Anorganic Bovine-Derived Hydroxyapatite vs β-Tricalcium Phosphate in Sinus Augmentation: A Comparative Histomorphometric Study
Peripheral Giant Cell Granuloma Associated With Dental Implants: Clinical Case and Literature Review
Bilateral Vertical Ridge Augmentation With Block Grafts and Guided Bone Regeneration in the Posterior Mandible: A Case Report
Cell Culture–Based Tissue Engineering as an Alternative to Bone Grafts in Implant Dentistry: A Literature Review

Journal of Oral Implantology September 2012

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