Journal of Oral Implantology April 2013 - (Page 154)

RESEARCH Fluoride-Treated Bio-Resorbable Synthetic Hydroxyapatite Promotes Proliferation and Differentiation of Human Osteoblastic MG-63 Cells Motofumi Ohno, DDS1 Kazunari Kimoto, DDS, PhD1* Toshihisa Toyoda, DDS, PhD2 Kazushige Kawata, DDS1 Hirohisa Arakawa, DDS, PhD1 When resorbable hydroxyapatite (HA) granules, which are used as a bone supplement material, were treated in neutral 4% sodium fluoride (NaF) solution, formation of a reactant resembling calcium fluoride was observed on the surface of the granules. Immediate and slow release of fluoride from fluoridated HA (HAþF) granules was observed after immersion in culture fluid, and the concentration increased over time to 1.25 6 0.05 ppm F at 0.5 hours, 1.57 6 0.12 ppm F at 24 hours, and 1.73 6 0.15 ppm F at 48 hours. On invasion assay, migration of human osteoblast-like MG-63 cells exposed to the released fluoride was confirmed in comparison to the cells incubated with a nonfluoridated control sample (P , .01). In addition, fluoride added to the medium increased MG-63 cell proliferation in a manner dependent on fluoride concentrations up to 2.0 ppm (P , .05). At 5.0 ppm, however, fluoride significantly inhibited cell proliferation (P , .005). Activity of the osteogenic differentiation marker, alkaline phosphatase (ALP), also increased with fluoride after exposure for 1 week, increasing significantly at 1.0 ppm (P , .05). The promotion of MG-63 cell migration and proliferation, as well as increased ALP activity, suggested that fluoride released from the surface of resorbable HA granules, which were fluoridated by prior treatment with neutral 4% NaF solution, can provide a superb method to supply fluoride and promote osteogenic cell differentiation. Key Words: fluoride slow release, bio-resorbable hydroxyapatite, cell migration, proliferation, ALP activity INTRODUCTION I mplants cannot be placed in deficient bone and pretreatments such as guided bone regeneration are necessary in such cases. Materials currently used for guided bone regeneration include autograft bone, allograft bone, xenogeneic bone, and artificial materials such as hydroxyapatite (HA) and tricalcium phosphate granules.1,2 1 Division of Oral Health, Department of Health Science, Kanagawa Dental College, Yokosuka, Japan. Tokyo Plastic Surgery Dental Society, Tokyo, Japan. * Corresponding author, e-mail: kimotokz@kdcnet.ac.jp DOI: 10.1563/AAID-JOI-D-10-00175 2 154 Vol. XXXIX /No. Two / 2013 In a previous study, fluorapatite was prepared from resorbable synthetic HA granules by treating them with 4% sodium fluoride (NaF) at neutral pH for 2 minutes. Rinsed powder was then subjected to analyses using electron microscopy, 1-dimensional and 2-dimensional X-ray diffraction, energy dispersive X-ray (EDX) analysis, and EDX mapping.3 We showed that the reaction of fluoride ions with the HA granules produced crystalline fluorapatite having characteristic and sharp X-ray diffraction patterns on the surface of granules. EDX analysis revealed a fluoride peak at 0.70 KeV that was not seen on the surface of nonfluoridated control granules. Furthermore, EDX mapping showed an evenly distributed needle crystalline particulate

Table of Contents for the Digital Edition of Journal of Oral Implantology April 2013

Evidence-Based Practice or Practice-Based Evidence?
Evaluation of Optimal Taper of Immediately Loaded Wide-Diameter Implants: A Finite Element Analysis
A Prospective, Randomized Controlled Preclinical Trial to Evaluate Different Formulations of Biphasic Calcium Phosphate in Combination With a Hydroxyapatite Collagen Membrane to Reconstruct Deficient Alveolar Ridges
Retention of Implant-Supported Overdenture With Bar/Clip and Stud Attachment Designs
The Clinical and Histologic Outcome of Dental Implant in Large Ridge Defect Regenerated With Alloplast: A Randomized Controlled Preclinical Trial
Fluoride-Treated Bio-Resorbable Synthetic Hydroxyapatite Promotes Proliferation and Differentiation of Human Osteoblastic MG-63 Cells
Resonance Frequency Analysis of 208 Straumann Dental Implants During the Healing Period
A Simplified Technique for Solving the Transfer Problem of Implant-Supported Fixed Partial Dentures for Patients With Microstomia
Esthetic Evaluation of Single-Tooth Morse Taper Connection Implants Placed in Fresh Extraction Sockets or Healed Sites
A Technique for Improving the Retention of Dentures Retained by Ramus Frame Implants
Treatment of Congenitally Missing Maxillary Lateral Incisors: An Interdisciplinary Approach
Use of Titanium Mesh in Lieu of a Fixation Screw to Stabilize an Autogenous Block Graft: A Case Report
A Novel Approach to Preserve the Buccal Wall in Immediate Implant Cases: A Clinical Report
Implant Failure Associated With Actinomycosis in a Medically Compromised Patient
Replacement of Mandibular Posterior Teeth With Implants in a Postmandibular Resection Case: A Case Report
Zygomatic Implants/Fixture: A Systematic Review
An Overview of Immediate Root Analogue Zirconia Implants
Letter to the Editor
Reviews of Current Literature

Journal of Oral Implantology April 2013