Journal of Oral Implantology August 2013 - (Page 433)

RESEARCH Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue Afshin Khorsand, DMD, MS1 Mohamadreza Baghaban Eslaminejad, PhD2* Mohadeseh Arabsolghar, DMD, MS3 Mojghan Paknejad, DMD, MS1 Baharak Ghaedi, DMD, MS4 Amir Reza Rokn, DMD, MS1 Neda Moslemi, DMD, MS1 Hamid Nazarian, MSc2 Shahrbanoo Jahangir, MSc2 This study aimed to investigate effects of dental pulp stem cells (DPSCs) on regeneration of a defect experimentally created in the periodontium of a canine model. Surgically created mesial 3-walled periodontal defects with ligatureinduced periodontitis were produced bilaterally in the first lower premolar teeth of 10 mongrel dogs. Simultaneously, DPSCs were derived from the maxillary premolar teeth of the same dogs. Four weeks after creation of the periodontitis model, autologous passaged-3 DPSCs combined with Bio-Oss were implanted on one side as the test group. On the other side, only Bio-Oss was implanted as a control. Eight weeks after surgery, regeneration of the periodontal defects was evaluated histologically and histomorphometrically in terms of bone, periodontal ligament (PDL), and cement formation. Histologically, in all test specimens (10 defects), regeneration of cementum, bone, and PDL was observed. In the control groups, although we observed the regeneration of bone in all defects, the formation of cementum was seen in 9 defects and PDL was seen in 8 defects. Histomorphometric analyses showed that the amount of regenerated cementum and PDL in the test groups (3.83 6 1.32 mm and 3.30 6 1.12 mm, respectively) was significantly higher than that of the control groups (2.42 6 1.40 mm and 1.7761.27 mm, respectively; P , .05). A biocomplex consisting of DPSCs and Bio-Oss would be promising in regeneration of periodontal tissues. Key Words: dental pulp stem cells, regeneration, periodontal defect, Bio-Oss INTRODUCTION P 1 eriodontitis is a complex disease of the periodontium that results in considerable defects to alveolar bone, gingival tissue, and periodontal ligament (PDL).1 Reconstruction of defects resulting from Dental Research Center, Periodontics Department, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 2 Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. 3 Department of Periodontology, School of Dentistry, Kerman University of Medical Sciences. Kerman, Iran. 4 Department of Periodontology, School of Dentistry, Hamedan University of Medical Sciences. Hamedan, Iran. * Corresponding author, e-mail: eslami@royaninstitute.org DOI: 10.1563/AAID-JOI-D-12-00027 periodontal disease is a major challenge to regenerative medicine in the oromaxillofacial field. On the other hand, in the field of tissue regeneration, the use of tissue-resident stem cells as regenerative materials has gained considerable attention owing to their self-renewal property and multilineage differentiation capacity. Among stem cells, dental stem cells are of interest as they are easily accessible and can be collected noninvasively with low morbidity.2 In this context, the periodontal ligament stem cell (PDLSC) has been successfully investigated in some animal models. Liu et al3 investigated the utility of autologous PDLSC to treat ligature-induced periodontitis in miniature swine. The 3-walled defect was generated in the first molar area of miniature Journal of Oral Implantology 433

Table of Contents for the Digital Edition of Journal of Oral Implantology August 2013

Literary Responsibility—Everyone Has a Role to Play
Rehabilitation of Surgically Relocated Integrated Dental Implants With and Without Bone Morphogenesis Protein-2
Erratum
Marginal Fit of Implant-Supported All-Ceramic Zirconia Frameworks
Influence of the Implant Diameter With Different Sizes of Hexagon: Analysis by 3-Dimensional Finite Element Method
Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue
Histologic and Biomechanical Evaluation of 2 Resorbable-Blasting Media Implant Surfaces at Early Implantation Times
Efficacy of Guided Bone Regeneration Using Composite Bone Graft and Resorbable Collagen Membrane in Seibert’s Class I Ridge Defects: Radiological Evaluation
Minimal Iliac Bone Harvesting in Sinus Lift Surgery
Functional Load in Oblique Bicortical Implants: Parasinusal Implants and Palatine Implants
Ultrasonic Oscillations for Conservative Retrieval of a Rare Fracture of Implant Healing Abutment
Craniofacial Implant-Retained Auricular Prosthesis: A Case Report
Gingival Conditioning in an Implant-Supported Prosthesis: A Clinical Report
An Early Loaded Implant-Supported Mandibular Complete Arch Fixed Prosthesis in a Young Completely Edentulous Patient: A Case Report
Computer Guided Surgery for Implant Placement and Dental Rehabilitation in a Patient Undergoing Sub-Total Mandibulectomy and Microvascular Free Flap Reconstruction
Guided Bone Regeneration for Socket Preservation in Molar Extraction Sites: Histomorphometric and 3D Computerized Tomography Analysis
Osseointegration of Dental Implants and Osteonecrosis of the Jaw in Patients Treated With Bisphosphonate Therapy: A Systematic Review
Review of Current Literature

Journal of Oral Implantology August 2013