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« DRUG DEVELOPMENT
* Alternatively, the bacterial cells are made permeable by
subjecting them to electrical pulses, a process known as
electroporation.
The transformation efficiency is defined as the number of
transformants generated per µg of supercoiled plasmid DNA used in
the transformation reaction.
What Are the Applications of
Transformation?
The phenomenon of transformation has been widely used in molecular
biology. As they are easily grown in large numbers, transformed
bacteria may be used as host cells for the following:
* make multiple copies of the DNA
* in cloning procedures
* express large amounts of proteins and enzymes
* in the generation of cDNA libraries
* in DNA linkage studies
What is Required in a Typical Transformation Reaction?
* Competent cells
* Supercoiled plasmid DNA
* Transformation medium
* Selection marker (antibiotic and/or chromogenic substrate)
References
1.
2.
3.
EudraLex - Volume 4 - Good Manufacturing Practice (GMP) guidelines, Part IV - GMP
requirements for Advanced Therapy Medicinal Products, https://ec.europa.eu/health/
documents/eudralex/vol-4_en
The Alliance for Regenerative Medicine Global Sector Report, 2019
Kenneth Lundstrom, Ph.D., Gene Therapy Applications of Viral Vectors, Technology in
Cancer Research & Treatment, ISSN 1533-0346, Volume 3, Number 5, October, 2004,
Adenine Press. https://journals.sagepub.com/doi/pdf/10.1177/153303460400300508
4. Westburg, Efficient mRNA transfection for gene expression of difficult-to-transfect cells.
https://www.westburg.eu/efficient-mrna-transfection-for-gene-expression-of-difficultto-transfect-cells#:
5.
6.
5.
Griffith, Fred. (January 1928). " The Significance of Pneumococcal Types " . Journal of
Hygiene. Cambridge University Press. 27 (2): 113-159. doi:10.1017/S0022172400031879.
JSTOR 4626734. PMC 2167760. PMID 20474956.
Avery, Oswald T. (1944-02-01). " Studies on the chemical nature of the substance
inducing transformation of pneumococcal types: induction of transformation by
a Desoxyribonucleic Acid fraction isolated from Pneumococcus Type III " . Journal of
Experimental Medicine. 79 (2): 137-158. doi:10.1084/jem.79.2.137. PMC 2135445.
PMID 19871359. Retrieved 2008-09-29.
www.americanpharmaceuticalreview.com |
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APR January/February 2022

Table of Contents for the Digital Edition of APR January/February 2022

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