May/June 2021 - 27

these wild potatoes that are resistant to
the Colorado potato beetle and ones that
are susceptible to the pest. Kaiser then
identified key changes at the DNA level
between the potatoes that explained the
differences in their chemical profile and
Colorado potato beetle likability.
Now that we know the suite of
genes required to make a potato plant
resistant, the next challenge is to
transfer all the genes into a potato
cultivar with other good characteristics,
such as high yield and desirable tuber
appearance, by cross pollinating. This
process is complicated by the fact that
potato cultivars have four copies of
every gene. To make matters more
complex, each of the four copies can
be a different version of the gene. It is
difficult to make sure that all four copies
of the genes we are interested in are the
desirable copy necessary for resistance.
One strategy to overcome this issue
is to work with potatoes that naturally
only have two copies of every gene,
which are known as diploid potatoes.
Many wild potato relatives, including
the Colorado potato beetle resistant
Figure 2. Colorado potato beetle larvae can be put on potato leaves in the lab to identify
resistant plants without planting a field trial. The wild potato Solanum chacoense 80-1,
which contains leptine glycoalkaloids, remained resistant to defoliation over the seven-day
experiment.
types that produce leptines, are diploids.
Through self-pollination, Kaiser has
been able to develop inbred diploid
lines with strong resistance to Colorado
potato beetle. These diploid inbred
lines simplify the breeding process
because the traits breed true, like the
two parents in a corn hybrid. We can
more easily track how genes impacting
beetle resistance are inherited because
we know the copy of each gene they will
pass along to their offspring.
Excitingly, these are the first inbred
lines selected for a specific resistance
2021 Buyers' Guide
Your year-round resource to industry suppliers
The 2021 Buyers' Guide
digital edition features a
comprehensive listing of
suppliers in every category for
the potato industry, making it
easy for you to search for what
you need all year long.
View the Buyers' Guide at
Spudman.com/BuyersGuide
Spudman * May/June 2021
27

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May/June 2021

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