eBook: Characterizing Extracellular Vesicles with Flow Cytometry - 9
A
1:60 dilution
Population: Potential EVs
B
1e+4
C
1:120 dilution
Population: Potential EVs
1e+4
APC Intensity
APC Intensity
APC Intensity
1e+4
1e+3
1e+3
1e+3
1:240 dilution
Population: Potential EVs
0
0
0
-500
-500
-500
-500
0
1e+3
-500
1e+4
0
1e+3
-500
1e+4
0
1:480 dilution
Population: Potential EVs
D
1e+3
1e+4
PE Intensity
PE Intensity
PE Intensity
1:960 dilution
Population: Potential EVs
E
1e+4
APC Intensity
APC Intensity
1e+4
1e+3
1e+3
0
0
-500
-500
-500
0
1e+3
1e+4
-500
0
1e+3
PE Intensity
1e+4
PE Intensity
Figure 2. Bivariate dot plots for the dilution series of RBC-EVs and platelet-EVs labeled with CD235ab-PE and CD41-APC, respectively.
PE+ events from Figure 1B are colored green, and APC+ events from Figure 1C are colored red. The dilutions are: (A) 1:60, (B) 1:120, (C)
1:240, (D) 1:480, and (E) 1:960.
PE+ and APC+ objects per μl for the various experimental and control samples are shown in Figure 4
(A, B): labelled EVs, antibody only, antibody + Triton® X-100, labelled EVs + Triton X-100, and buffer
only. The objects per μL are the events in the PE+ or
APC+ gates shown in Figure 1. Tables 1 and 2 show
the average objects per μl and standard deviations
for the PE+ and APC+ events in Figures 4A and 4B,
respectively.
Summary
In this study, EVs derived from RBCs and platelets were immunophenotyped on the CellStream
9
eBook: Characterizing Extracellular Vesicles with Flow Cytometry
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Contents
eBook: Characterizing Extracellular Vesicles with Flow Cytometry - 1
eBook: Characterizing Extracellular Vesicles with Flow Cytometry - Contents
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