Contents 4 Challenges in cfDNA Sample Preparation Find out how to extract as much relevant diagnostic data as possible from fragmented, low-level nucleotides. 7 What's New in Nucleic Acid Extraction Whether the approach used is manual or automated, a number of updates can help make extractions more successful. 10 Avoid Bottlenecks in High-Throughput NGS Sample Prep A comparison of approaches to accelerate NGS sample preparation and their amenability to automation. 16 Unraveling the Challenges of Nucleic Acid Isolation DNA and RNA extraction for a variety of common sample types. 20 Nucleic Acid Isolation from Clinical Samples Addressing the challenges of utilizing fresh frozen tissue samples, FFPE tissue blocks, and liquid biopsy for NGS. 28 Resources 2