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PID controlled Constant RQ Fermentation of Pichia pastoris in the DASbox® Mini Bioreactor System
PID controlled Constant RQ
Fermentation of Pichia pastoris in
the DASbox® Mini Bioreactor System
Nina Schrand1, Malte Schneider1, Ma Sha2
1 Eppendorf SE, Bioprocess Center, Juelich, Germany
2 Bioprocess Applications Lab, Eppendorf, Inc., Enfield, CT, USA
Contact: bioprocess-experts@eppendorf.com
Abstract
T
he yeast Pichia pastoris is largely used for
heterologous protein production in biotechnology.
For optimizing the production yield,
one of the most important factors is the feeding
strategy. One of the leading strategies for protein
production-oriented feeding is based on respiratory
12 | GENengnews.com
quotient (RQ). Constant RQ-based feeding ensures
that the respiratory metabolism of glucose/glycerol
are optimized for protein production purposes and
the formation of by-products are limited [1].
To demonstrate the feasibility of constant respiratory
quotient (RQ) based feeding via a script integrated
to the DASware® control 5 bioprocess control software,
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EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency

Table of Contents for the Digital Edition of EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency

Contents
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 1
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 2
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - Contents
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EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 5
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 6
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 7
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 8
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 9
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 10
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 11
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 12
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 13
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 14
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 15
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 16
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