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UPSTREAM BIOPROCESSING: IMPROVING EFFICIENCY THROUGH DIGITAL TOOLS
Figure 1: A. Exponential feed function B. Part of a script to apply exponential feed profiles for two feeds. The execution
of the script starts 12 hours after inoculation. For its initiation, the script accesses the time after inoculation start, which
is automatically recorded by the software. During this time, the script controls the flows of the pumps. Ten hours after
initiation of the script the flow rate of the pumps is set to zero to end feeding.
execution of the scripts. Figure 1B shows a part of a
script programmed to apply an exponential feed and
explains the system parameters it uses.
Exponential feeding of an actively growing culture
aims at elongating the exponential growth phase for
higher biomass density, while effectively avoiding
nutrient depletion and toxic byproduct buildup.
Strategy 2: Sensor-based feeding
In a sensor-based feeding strategy, a feed solution is
added to the culture based on a sensor reading.
Figure 2 describes such an automated feedback loop:
Figure 2: Automated feedback loop
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EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency

Table of Contents for the Digital Edition of EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency

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EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 1
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 2
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - Contents
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EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 5
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 6
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 7
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 8
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 9
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 10
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 11
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 12
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 13
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 14
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 15
EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 16
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EPPENDORF_Nov2021_UpstreamBioprocessingImprovingEfficiency - 18
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