Sartorius_May21_NeutAntiMakingTheir - 19

NEUTRALIZING ANTIBODIES MAKING THEIR MARK IN NEXT WAVE OF BIOLOGICS

in patient samples. In collaboration with

advanced screening methods that incorporate a

colleagues at Mt. Sinai, researchers at LifeArc

novel, innovative reagent for characterizing ABI

produced 1,152 candidate hybridoma clones

via both advanced flow cytometry and live-cell

that they were able to narrow to 53 candidates

imaging, they gained maximum insight through

that ELISA and flow cytometry showed bound

high-content data and were able to make critical

to ALK. Using the advanced flow cytometry

decisions early in their process, thus saving time,

capabilities of the iQue® platform, the

material, and money. n

researchers were able to further narrow this to
20 candidates that bound ALK at the surface

References

of cells, since this is a critical attribute of the

Tiller KE and Tessier PM. Advances in antibody design. Annu Rev Biomed
Eng, Feb 5; 17: 191-216 (2015) PMID: 26274600

mechanism of action of ADCs.
Of particular interest, these researchers used
internalization assays on both the iQue® and
Incucyte cell analysis platforms to further narrow
their lead candidates to two. Critically, they
gained kinetic imaging data and high- content
analysis using multiplexed cell viability assays
early in their ADC discovery process. Using

Doerner A, et al. Therapeutic antibody engineering by high efficiency
cell screening. FEBS Lett, Jan 21; 588(2); 278-87 (2014) PMID: 24291259
Nath N, et al. Homogeneous plate based antibody internalization
assay using pH sensor fluorescent dye. J Immun Meth, Feb 3; 431: 11-21
(2016) PMID: 26851520
Reidl T, et al. High-Throughput Screening for Internalizing Antibodies
by Homogeneous Fluorescence Imaging of a pH-Activated Probe. J
Biomol Screen, Jan; 21(1): 12-23 (2016) PMID: 26518032
Chames P, et al. Therapeutic antibodies: successes, limitations and
hopes for the future. Br J Pharmaco, May; 157(2): 220-33 (2009) PMID:
19459844
Saeed A, et al. Antibody Engineering for Pursuing a Healthy Future.
Front Microbiol, Mar; 8(495) (2017) PMID: 28400756

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