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Octet® Bio-Layer Interferometry Systems

antibodies and other recombinant proteins for

and epitope binning assays. See full selection of

screening without the risk of sample clogging.

biosensor chemistries.

These features enable users to easily vary assay

Octet® instruments have been widely

and biosensor conditions and reuse precious

deployed in various vaccine development

samples that result in fast and low-cost assay

research programs (HIV1,2, influenza3, Ebola4,

optimization.

novel coronavirus5-15) and in evaluating vaccine

Additionally, users can choose from a range

agents such as nanoparticles16, virus-like

of tailored affinity capture and immobilization

particles (VLPs)17 and bispecific antibodies18.

chemistries suitable for vaccine and

Octet® systems also provide solutions beyond

biotherapeutic development research. Widely

lead candidate selection to bioprocess

used, ready-to-use biosensor chemistries are

development and manufacturing. This review

listed in Table 1.

summarizes the use of Octet® assays in some

Table 1: Widely used biosensor chemistries for
antibody-based kinetic analysis, receptor binding

recent advances in coronavirus research and
vaccine development efforts.

Table 1: Widely used biosensor chemistries for antibody-based kinetic analysis, receptor binding and epitope binning assays. See full
selection of biosensor chemistries.

Biosensor

Description

AHC

Anti-Human Fc-Capture

Capturing human IgGs or human Fc-fusion proteins from purified or unpurified
media for kinetic or epitope binning analysis with various analytes.

AMC

Anti-Mouse Fc-Capture

Capturing mouse IgGs or mouse Fc-fusion proteins from purified or unpurified
media for kinetic or epitope binning analysis with various analytes.

SA

Streptavidin

Immobilizing biotinylated molecules for all kinetic or epitope binning analysis.

HIS1K

Anti-Penta-HIS

Capture of His-tagged proteins from purified or unpurified media for kinetic analysis with target analytes. Quantitation of His-tagged proteins in buffer, media or
diluted lysate. Biosensor is pre-coated with Penta-His antibody.

FAB2G

Anti-Human Fab-CH1
2nd Generation

Kinetic analysis of human Fab fragments and IgG with target antigen, Fc receptors,
or other analytes. Quantitation of Fab and IgG.

NTA

Ni-NTA

Protein A

Fc-Capture

32 |

GENengnews.com

Application

Quantitation of HIS-tagged proteins in buffer or diluted matrix, capturing of HIStagged proteins for kinetic analyses with various analytes.
Capturing IgGs and Fc fusion proteins from various species including human.


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