Sartorius_May21_NeutAntiMakingTheir - 40

Octet® Bio-Layer Interferometry Systems

Table 2: Examples of Octet® BLI assays in coronavirus research and vaccine development.

Analyte(s)

Immobilized ligand(s)

Virus
species

Assay focus

Biosensor

Reference

ACE2

Fc-tagged SARS
CoV-2 RBD

SARS
CoV-2

Cross-reactivity and receptor
binding

AHC

12

ACE2

SARS-CoV-2 SB
SARS-CoV SB

SARS
CoV-2

Receptor binding

HIS1K

10

mAb, MERS S protein, VHH

SARS CoV-2 S1,
SARS CoV-2 RBD

SARS
CoV-2

VHH and mAb binding assessment,
Epitope binning

AR2G

13

SARS
CoV-2

FAB, IgG binding characterization

HIS1K

14

SARS
CoV-2

Cross-reactivity binding

SA

9

SARS
CoV-2

Neutralization antibody assessment

SA

8

AHC, HIS1K

5

Fab, IgG
ACE2, SARS-CoV
RBD
Antibodies

SARS CoV-2 S protein, SARS CoV-2
RBS
SARS-CoV-specific
neutralizing antibodies
Biotin-Recombinant , SARS-CoV S1
protein

FAB, ACE2, SARS S
protein

mAb, ACE2

SARS
CoV-2

Receptor binding, antibody reactivity, competition assays

SARS CoV-2 RBD

Inhibitor Peptide

SARS
CoV-2

Peptide inhibitor development

SA

15

mAb

S1B and SARS Secto
domains

Antibody reactivity assessment

HIS1K

11

SARS CoV-2 RBD,
SARS CoV RBD,
mAbs, FAB

mAbs, SARS CoV-2
RBD, SARS CoV RBD

Protein A,
HIS2, HIS1K

7

Coronavirus S
proteins

Biotin-9OAc6SLN

Receptor binding

SA

20

MERS-5HB fusion
inhibitor

Biotin-peptide
(MERS-HR2P)

MERS CoV

Protein Inhibitor development

SA

21

mAb, MERS S
protein

mAb, MERS S
protein

MERS CoV

Epitope binning and Receptor
binding

SAX, Protein
A

22

MERS-CoV S

mAbs

MERS CoV

Antibody reactivity measurements,
competition assays

AHC, HIS1K

23

MERS-CoV NTD,
mutants

mAb

MERS CoV

Antibody reactivity measurements

AHC

24

MERS S-protein

mAbs

MERS CoV

Antibody reactivity measurements

AHC

25

RBD/S1/S2 antigen

Vaccine-induced
mouse
monoclonal IgGs

MERS CoV

Antibody reactivity and epitope
binning

AHC, HIS1K

26

FABs

MERS-CoV RBD

MERS CoV

Epitope binning

HIS1K

27

40 |

GENengnews.com

SARS CoV
and SARS
CoV-2
SARS CoV
and SARS
CoV-2
SARS CoV
and MERS
CoV

Antibody binding characterization,
epitope binning, competition
binding


http://www.GENengnews.com

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