Perfusion Medium Considerations How to Choose a Perfusion Medium T here are many factors to consider when choosing a perfusion medium. Two such factors are ease of use and cost-effective performance. Related to ease of use, consider what is a reasonable shelf life and what the reconstituted shelf life should be to provide you with flexibility when preparing and storing the medium. Additionally, and especially when it comes to the larger volumes of medium generally needed for perfusion work, it is important for the medium format to be easy to work with. The Gibco™ Advanced Granulation Technology™ (AGT™) format supports fast and simple reconstitution. This format saves time and reduces the number and complexity of steps, and common errors when reconstituting the medium. No matter the format, the formulation should be functional as both a seed train medium and a production medium capable of supporting high cell densities and productivity at lower medium exchange rates, ideally as low as 1 vessel volume per day (VVD). Lastly, the medium should support cost-effective scale-up.