ThermoFisherScientific_Jan2021_ADeepDiveInto - 18

Perfusion Medium Considerations
When comparing run conditions, it is important to
consider cell size. Figure 5 normalizes run data with
cell size, showing viable cell volume (solid lines)
and productivity per viable cell volume (dashed
lines). Looking at the viable cell volume being
maintained between the two conditions, it can be
seen that the decline in growth rate (used to determine when to start the bleed) occurred at a similar
viable cell mass. Looking at productivity as milligrams of product per viable cell volume, the two
conditions are almost perfectly in alignment during
the entire process.

Additional Resources
For additional resources, go to
thermofisher.com/perfusion.

It was clearly demonstrated
that the perfusion medium can
generate a stable process in a
range of concentrations.

Viable cell volume
(mm3/mL)

CHO K1 66% LGPM VCD
CHO K1 LGPM VCD

100
90
80
70
60
50
40
30
20
10
0

100

66% LGPM Qp
LGPM Qp

80
60
40
20
0

Days from inoculation

18 | GENengnews.com

Qp
(mg/cm3 viable cell volume)

Both conditions demonstrated successful operation
with HIP CHO Medium and maintained high steadystate percent viability and stable production. It was
clearly demonstrated that the perfusion medium can
generate a stable process in a range of concentrations.
This allows use of higher concentrations to push for
lower VVD and higher cell density and titer, or lower
concentrations to support cell lines that need leaner
medium or intentionally higher VVD. n

Figure 5. Viable cell volume
(viable cells/mL x 4/3 x π
x (average cell radius)3) is
plotted along with " true "
volumetric productivity (mg
product/viable cell volume).
Note: Units for Qp shown in
this graph are different from
typical Qp units based on VCD.

https://www.genengnews.com https://www.thermofisher.com/us/en/home/life-science/bioproduction/gibco-bioprocessing/cho-media/gibco-perfusion-medium.html?cid=fl-perfusion https://www.genengnews.com

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