ThermoFisherScientific_Jan2021_ADeepDiveInto - 22

Best Practices for Evaluating a Perfusion Medium

Know Your Cell Line
It is important to know the limits of your cell line.
Some of this can be built into your screening test
model as noted previously. Some limitations need to
be evaluated and considered directly. For example,
some cell clones lose productivity too quickly when
selective pressure is removed to be able to support a
long-term continuous perfusion process. Some cell
clones do not behave well at higher VCD, which may
limit perfusion benefits. Figure 2 is from a cell line
stress run with ramping agitation up to 600 rpm. The
cell line handled the stress run and other mechanical
stress tests well, but the production stability made it a
poor candidate for continuous perfusion tests.
Another limitation to cell line development could
be the selection of the medium it was developed in.
Before you begin your test, make sure your cell line
is fully adapted to any medium conditions. Keep in
mind that some cell lines require complex or specialty
components from the medium they were developed
in. Make sure these are present in all medium conditions tested. Failure to include special components
such as growth factors, peptones, and hydrolysates

Keep in mind that some cell lines
require complex or specialty
components from the medium
they were developed in.

100

Viability (%)

95

600

90

Viability

85

Agitation speed

500
400

Antifoam conc.

80

Total gas

300

75

200

70

100

65
60

0

10

20

30

40

50

Agitation speed (rpm)
Antifoam conc. (g/L) x 100
Total gas (mL/min)

700

0

Days

Figure 2. Chemostat operation of a CHO cell culture evaluating varying mechanical stress factors while monitoring cell health.
22 | GENengnews.com


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ThermoFisherScientific_Jan2021_ADeepDiveInto

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