Best Practices for Evaluating a Perfusion Medium understand which performance assumptions are reasonable and which risks are practical. When evaluating a perfusion process and the medium to be used, keep the following key points in mind: * Have a clear goal and design space determined for your screening so that you can build on top of your data and be confident in your comparisons. * Make sure your test model and equipment are robust enough to deliver the performance you are looking for within your design space and don't accidentally limit behaviors. * Understand your cell line's needs prior to developing a screening model and make sure the formulation comparisons are complete before adapting the model. * Know and account for the limits in what your screening model doesn't address and plan for validation runs to help ensure your assumptions are reasonable. n Make sure your test model and equipment are robust enough to deliver the performance you are looking for within your design space and don't accidentally limit behaviors. Additional Resources For additional resources, go to thermofisher.com/perfusion. 200 100% 160 95% 140 120 90% 100 80 85% 60 40 80% 20 0 0 5 10 15 20 25 30 35 40 75% Days Figure 3. Continuous perfusion operation at 1 VVD targeting constant cell viability, run in triplicate. Dotted lines are ±1 standard deviation. Note: harvest titer is in units of 10 mg/L. 24 | GENengnews.com Cell viability (%) VCD (x 10 6/mL) 10 mg/L harvest concentration 180https://www.genengnews.com https://www.thermofisher.com/us/en/home/life-science/bioproduction/gibco-bioprocessing/cho-media/gibco-perfusion-medium.html?cid=fl-perfusion https://www.genengnews.com