Agilent 2019 - 10

Automated Nucleic Acid Sample Quality Control in NGS Workflows

Final library QC

Adapter-ligated samples were processed
according to the SureSelectXT protocol, including
hybridization and capturing, followed by indexing
and amplification. In general, final libraries are
equimolar pooled for sequencing, which requires
a QC step to ensure accurate and precise
evaluation of library size and quantity. The libraries
were analyzed with the HS DNA assay (2100
Bioanalyzer system) and with the HS D1000
ScreenTape assay (4150 TapeStation system).
Electropherogram patterns with a broad single
peak and the absence of artifacts represent
high-quality libraries. All samples depicted
high-quality libraries, example electropherograms
are shown in Figure 5.

FU

A

200

150

100

50

r
pe
Up

60
0

20
0

w
Lo

Sample intensity (normalized FU)

500

Size (bp)

10,380

2,000

1,000

600

500

400

300

200

150

100

er

35

0

B

400

300

200

100

10

| GENengnews.com

1,000
1,500

700

500

400

300

200

100

50

25

0

Size (bp)

Figure 5. Example electropherograms of end products of
the SureSelectXT workflow. A) Final library analyzed with the
Agilent HS DNA assay, B) Sample analyzed with the Agilent
HS D1000 ScreenTape assay.



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Agilent 2019

Table of Contents for the Digital Edition of Agilent 2019

Contents
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