Agilent 2019 - 11

Automated Nucleic Acid Sample Quality Control in NGS Workflows

450

The electropherogram pattern of DNA smears and
libraries can differ between the 4150 TapeStation
system and the 2100 Bioanalyzer system due to
differences in technology. Nevertheless, resulting
sizing data was highly comparable between both

11

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HS D1000 ScreenTape assay

350
300
250
200
150
100
50

Both systems provide reliable results for final
library size determination.

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Sample number

Figure 6. Sizing comparison of the final
libraries generated with the Agilent
SureSelectXT workflow. The size was
determined by region analysis ranging
from 200 to 600 bp. Sizing data was
determined with the Agilent HS DNA
assay and compared to the Agilent HS
D1000 ScreenTape assay.

30
Qubit dsDNA HS assay
HS DNA ScreenTape assay
25

Concentration (ng/µL)

Alacris Theranostics uses the average size
provided by the TapeStation analysis software and
the concentration determined with the Qubit assay
to determine molarity data. The concentration
data provided by the HS D1000 ScreenTape was
compared to the quantification results attained
from the Qubit dsDNA HS assay (Figure 7). The
quantification with HS D1000 ScreenTape assay
was highly comparable to Qubit results with an
average deviation of 11.1%.

HS DNA assay

400

Average size (bp)

The maximum peak size ranged from 350 to 400
bp. Due to the increased fragment size that was
aimed during shearing, the expected average
size ranges from 325 to 400 bp. To determine
the average size for molarity calculation, a region
was set ranging from 200 to 600 bp. The sizing
results of the HS D1000 ScreenTape assay were
compared to the results determined by the HS
DNA assay with the 2100 Bioanalyzer system.
The results were highly comparable for all samples
with an average deviation of 0.9% (Figure 6).

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Sample number

Figure 7. Quantification of SureSelectXT
final libraries with the Qubit dsDNA HS
assay compared to the Agilent HS D1000
ScreenTape assay. The samples were
diluted to match the Agilent HS D1000
ScreenTape assay range. The graph
shows the original library concentration.


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Agilent 2019

Table of Contents for the Digital Edition of Agilent 2019

Contents
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