Agilent 2019 - 19

Automated Nucleic Acid Sample Quality Control in NGS Workflows

The molarity data was directly compared to
the data of the same samples analyzed with
the DNA 1000 and HS DNA assays of the 2100
Bioanalyzer system. The results showed excellent
correlation for molarity analysis of sheared DNA
with the 4200 TapeStation system compared to
the 2100 Bioanalyzer system (Figure 1).

19

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y = 1.1004x
R² = 0.9987

700
600
500
400
300
200
100
0

0

100 200 300 400 500 600 700 800
Molarity D1000
ScreenTape assay (nmol/L)

Molarity
High Sensitivity DNA assay (pmol/L)

A dilution series of sheared DNA was analyzed
with the D1000 and HS D1000 ScreenTape
assays on the 4200 TapeStation system. A ladder
was run on each ScreenTape to ensure the most
accurate sizing.

A

Molarity
DNA 1000 assay (nmol/L)

lower and upper marker of an assay.
Table 1 shows that the quantitative range for
DNA smears differs from the quantitative range
for DNA fragments.

×104
5
B

800

y = 1.0042x
R² = 0.9992

4

3

2

1

0

0

1

2

3

4

Molarity High Sensitivity D1000
ScreenTape assay (pmol/L) ×104

Figure 1. Correlation of molarity data of sheared DNA samples in three concentrations (n = 6). The results of the 4200
TapeStation system are plotted on the X-axis and the results obtained with the 2100 Bioanalyzer system on the Y-axis.
The molarity was evaluated using region functionality. A) Sample molarities obtained with the D1000 ScreenTape assay
compared with molarity data from the DNA 1000 assay. B) Correlated molarity data of sheared DNA samples analyzed with
the HS D1000 ScreenTape assay and the HS DNA assay.

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Agilent 2019

Table of Contents for the Digital Edition of Agilent 2019

Contents
Agilent 2019 - 1
Agilent 2019 - 2
Agilent 2019 - 3
Agilent 2019 - 4
Agilent 2019 - Contents
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