Agilent 2019 - 30
Automated Nucleic Acid Sample Quality Control in NGS Workflows
A 70
The dilution series showed that DV200 evaluation
is most precise when measuring above the lower
limit of the specified assay concentration range,
data not shown.
60
60
55
55
DV200 (%)
65
50
45
50
45
40
40
35
35
30
30
61
61
CV (%)
3.0
0.5
Sample 6
Average
DV200 (%)
Sample 5
Sample 1
A dilution series for three individual FFPE RNA
samples was analyzed with the RNA and High
Sensitivity RNA ScreenTape assays to determine
the consistency of the DV200 analysis (Table 1).
Both the RNA ScreenTape and High Sensitivity
RNA ScreenTape assay yielded comparably high
DV200 precision of less than 5 % CV.
65
Sample 4
Concentration Dependence
70
Sample 3
A set of three FFPE RNA samples, kindly provided
by the tissue bank of the National Center for
Tumor Diseases (NCT, Heidelberg, Gemany) with
five individual replicates was used to verify the
reproducibility of the DV200 with the RNA and the
High Sensitivity RNA ScreenTape assay. Both
assays showed high reproducibility with a CV
of less than 5 % (Figure 2).
DV200 (%)
Reproducibility
B
Sample 2
Results and Discussion
59
Average
DV200 (%)
40
36
51
0.6
CV (%)
3.9
2.6
1.8
Figure 2. Reproducibility of DV200 analysis for three individual samples with (A) the Agilent RNA ScreenTape assay and
(B) the Agilent High Sensitivity RNA ScreenTape assay.
Agilent RNA ScreenTape assay
Agilent High Sensitivity RNA ScreenTape assay
Sample 7
Sample 8
Sample 9
Sample 10
Sample 11
Sample 12
Average DV200 in %
46
52
42
57
66
64
Standard deviation
1.1
1.6
1.7
1.6
0.6
0.9
% CV
2.4
3.1
4.1
2.8
1.0
1.4
Table 1. Consistency of DV200 analysis with the Agilent RNA and the Agilent High Sensitivity RNA ScreenTape assay
measured in dilution series for three samples per assay.
30
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Agilent 2019
Table of Contents for the Digital Edition of Agilent 2019
Contents
Agilent 2019 - 1
Agilent 2019 - 2
Agilent 2019 - 3
Agilent 2019 - 4
Agilent 2019 - Contents
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