Agilent 2019 - 9

Automated Nucleic Acid Sample Quality Control in NGS Workflows

systems correlated highly with an average
deviation of 2.2% (Figure 3).

350

Qualification of precapture libraries

300

DNA 1000 assay

Average size (bp)

D1000 ScreenTape assay

250
200
150
100
50
0

1

2

3

4

5

6

7

8

9

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Sample number

450

D1000 ScreenTape assay

350
300
250
200
150
100
50
0

9

| GENengnews.com

Figure 3. Average size of 30 sheared
DNA samples with a region set from
100 to 1000 bp. The average size was
determined with the Agilent DNA 1000
assay and the Agilent D1000 ScreenTape
assay.

DNA 1000 assay

400

Average size (bp)

The next steps for sheared DNA in the
SureSelectXT protocol are comprised of end repair,
adenylation, and adapter ligation followed by
amplification. To verify efficient adapter ligation,
purified adapter ligated DNA samples (precapture
samples) were qualified with the DNA 1000
assay (2100 Bioanalyzer system) and the D1000
ScreenTape assay (4150 TapeStation system).
The precapture libraries were analyzed by region
analysis with boundaries set from 200 to 700 bp.
The average region size ranged from 340 to 420
bp with a maximum peak size at approximately
300 bp. All samples showed an expected size shift
(approximately 30 bp) with regards to the sheared
DNA samples, which is related to adapter ligation.
The average size determined by both systems
was highly comparable for all 30 samples with
an average deviation of 2.7% (Figure 4). Hence,
the average region size of libraries can be
confidentially assessed using the region
functionality with the DNA 1000 assay as
well as with the D1000 ScreenTape assay.

400

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3

4

5

6

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9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
Sample number

Figure 4. Comparison of the average size
of 30 precapture libraries with a region
set from 200 to 700 bp. The average
size was determined with the Agilent
DNA 1000 assay and the Agilent D1000
ScreenTape assay.



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Agilent 2019

Table of Contents for the Digital Edition of Agilent 2019

Contents
Agilent 2019 - 1
Agilent 2019 - 2
Agilent 2019 - 3
Agilent 2019 - 4
Agilent 2019 - Contents
Agilent 2019 - 6
Agilent 2019 - 7
Agilent 2019 - 8
Agilent 2019 - 9
Agilent 2019 - 10
Agilent 2019 - 11
Agilent 2019 - 12
Agilent 2019 - 13
Agilent 2019 - 14
Agilent 2019 - 15
Agilent 2019 - 16
Agilent 2019 - 17
Agilent 2019 - 18
Agilent 2019 - 19
Agilent 2019 - 20
Agilent 2019 - 21
Agilent 2019 - 22
Agilent 2019 - 23
Agilent 2019 - 24
Agilent 2019 - 25
Agilent 2019 - 26
Agilent 2019 - 27
Agilent 2019 - 28
Agilent 2019 - 29
Agilent 2019 - 30
Agilent 2019 - 31
Agilent 2019 - 32
Agilent 2019 - 33
Agilent 2019 - 34
Agilent 2019 - 35
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