Bio-Rad eBook - 2019 - 5
Rapid Transfer Systems
Types of Blotting Systems
Cathode (-)
Filter Paper
There are three types of transfer systems:
1. Traditional wet tank transfer systems-gel and membrane
are submerged under transfer buffer in tank
2. Semi-dry transfer systems-gel and membrane are
sandwiched between buffer-wetted filter paper in direct
contact with electrodes
3. Rapid transfer systems-use specialized materials and
buffers optimized for rapid and efficient protein transfer
Gel
Membrane
Filter Paper
Anode (+)
Gel and Membrane Setup
for Electrophoretic Transfer
Pros and Cons of Wet Tank, Semi-Dry, and Rapid Blotting Systems
Tank systems can be used for transfers of proteins of all sizes; they allow for a flexible range of power and time
settings. On the downside, they require some manual assembly and typically require cooling. Transfer times are
long-30 minutes to overnight.
Semi-dry systems are typically easier to set up than tank systems and can complete transfer in 15-60 minutes.
They don't require cooling, but power and transfer time options are limited. They perform best for proteins in the
range of 30-120 kD.
Rapid transfer systems are a more recent development. These systems utilize specialized buffers and filter papers
and come pre-wetted and sealed in single-use packages, simplifying assembly of the transfer stack. They can
be used to transfer proteins over a broad size range and don't require cooling. Rapid transfer systems are very
efficient and can complete transfer in only 3 -10 minutes.
The Trans-Blot Turbo
Rapid Blotting Transfer System
The Trans-Blot Turbo Transfer System is a highperformance western blotting transfer system designed
to provide rapid transfers with high efficiency. The system
enables blot transfer of protein in as little as 3 minutes,
with performance comparable to traditional tank transfer.
Trans-Blot Turbo Transfer Packs include an optimized
buffer, membrane, and filter paper combination that
provides superior blot transfers.
bio-rad.com/TransBlot
Six Tips for Efficient Protein Transfer
bio-rad.com/6TransferTips
5
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Bio-Rad eBook - 2019
Table of Contents for the Digital Edition of Bio-Rad eBook - 2019
Contents
Bio-Rad eBook - 2019 - 1
Bio-Rad eBook - 2019 - 2
Bio-Rad eBook - 2019 - Contents
Bio-Rad eBook - 2019 - 4
Bio-Rad eBook - 2019 - 5
Bio-Rad eBook - 2019 - 6
Bio-Rad eBook - 2019 - 7
Bio-Rad eBook - 2019 - 8
Bio-Rad eBook - 2019 - 9
Bio-Rad eBook - 2019 - 10
Bio-Rad eBook - 2019 - 11
Bio-Rad eBook - 2019 - 12
Bio-Rad eBook - 2019 - 13
Bio-Rad eBook - 2019 - 14
Bio-Rad eBook - 2019 - 15
Bio-Rad eBook - 2019 - 16
Bio-Rad eBook - 2019 - 17
Bio-Rad eBook - 2019 - 18
Bio-Rad eBook - 2019 - 19
Bio-Rad eBook - 2019 - 20
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