Bio-Rad eBook - 2019 - 9
Stain-Free Gel and Blot Imaging
Validating Protein Transfer
One way to determine if the proteins in your gel were completely transferred to the membrane is by total protein
staining. Unfortunately, the use of total protein stains can interfere with subsequent blot development and
visualization steps, typically requiring tedious staining and destaining procedures.
Stain-Free Imaging
Stain-Free Imaging technology uses a proprietary trihalo compound that is cast into the acrylamide gel that
covalently binds to tryptophan residues in the sample upon brief UV activation. After transfer, images of the gel or
membrane can easily be captured multiple times without staining and destaining steps. This allows visualization,
verification, and validation at all steps of electrophoresis and blotting, saving time wasted on western blots with
problems that would not otherwise have been detected until the later stages of blot processing and development.
Verification of Western Blotting Protein Transfer Using Stain-Free Imaging
Gel before Transfer
Membrane after Transfer
Gel after Transfer
Assessment of protein transfer using a Stain-Free enabled imaging system. Images of the gel before and after transfer and of
the membrane after transfer were taken using a stain-free enabled imager. Serial 1:2 dilutions of hemoglobin (starting quantity, 80 ng),
with 1.8 μg of BSA/lane as a carrier (top band), were electrophoretically separated on a 4-20% 26-well Criterion Stain-Free gel.
Stain-Free Western Workflow
Bio-Rad's Stain-Free Western Workflow streamlines
your western blotting protocol, providing greater speed
and validation at each step of a western blotting experiment
-from running gels to quantitating proteins.
The complete Stain-Free Western Workflow incorporates
innovative tools including our proprietary stain-free gels
and the ChemiDoc MP Imaging System.
bio-rad.com/WesternWorkflow
Stain-Free Imaging Technology
bio-rad.com/StainFree
9
https://www.bio-rad.com/WesternWorkflow
https://www.bio-rad.com/stainfree
Bio-Rad eBook - 2019
Table of Contents for the Digital Edition of Bio-Rad eBook - 2019
Contents
Bio-Rad eBook - 2019 - 1
Bio-Rad eBook - 2019 - 2
Bio-Rad eBook - 2019 - Contents
Bio-Rad eBook - 2019 - 4
Bio-Rad eBook - 2019 - 5
Bio-Rad eBook - 2019 - 6
Bio-Rad eBook - 2019 - 7
Bio-Rad eBook - 2019 - 8
Bio-Rad eBook - 2019 - 9
Bio-Rad eBook - 2019 - 10
Bio-Rad eBook - 2019 - 11
Bio-Rad eBook - 2019 - 12
Bio-Rad eBook - 2019 - 13
Bio-Rad eBook - 2019 - 14
Bio-Rad eBook - 2019 - 15
Bio-Rad eBook - 2019 - 16
Bio-Rad eBook - 2019 - 17
Bio-Rad eBook - 2019 - 18
Bio-Rad eBook - 2019 - 19
Bio-Rad eBook - 2019 - 20
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